Title page for etd-0805114-103028


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URN etd-0805114-103028
Author Hsuan-Yi Hsiao
Author's Email Address No Public.
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Department Institute of Biomedical Sciences
Year 2013
Semester 2
Degree Master
Type of Document
Language English
Title Exercise protein irisin promotes angiogenesis in endothelial cells
Date of Defense 2014-07-04
Page Count 63
Keyword
  • Angiogenesis
  • white adipose
  • brown adipose
  • FNDC5 (Fibronectin type III domain-containing protein 5)
  • endothelial cells
  • Abstract Myokinesis a critical cytokines in organism major secretes from muscle cells after doing exercise. Myokines involved in angiogenesis, tissue regenerate and repair, preserved normal functions of body, affected immune modulation and develop of embryos. Boströmet al. found a new myokine and called FNDC5 in 2012. FNDC5 was founded possessing a ability that can induce White Adipose Tissue becoming Brown Adipose Tissue to lose weight and slow down the symptom of diabetes through increase burning calories. In the resent reports, there is no direct describe that whether FNDC5 can regulates angiogenesis and wound healing. Recombinant FNDC5 (Fibronectin type III domain-containing protein 5) was expressed and purified from E. coli with a molecular weight of 25 kDa. By using cultured endothelial cells, it was found that recombinant FNDC5 stimulated the proliferation, migration and tube formation of endothelial cells. Moreover, FNDC5 promotes vessels sprouting in aortic rings assay and in transgenic Tg(fli-1:EGFP)y1zebrafish. Finally, FNDC5 application enhanced wound healing in diabetic rats. Mechanism studies showed FNDC5 induced the expression of vascular the endothelial growth factor (VEGF)/vascular endothelial growth factor receptor 2 (VEGFR2/FLK-1) pathway and activated the downstream Erk and p38 signaling in endothelial cells. Therefore, this study unveils the novel pro-angiogenic the function and mechanism of FNDC5 in endothelial cells.
    Advisory Committee
  • Hung-Wen Huamg - chair
  • Chang-Yi, Wu - co-chair
  • Po-Lin Kuo - co-chair
  • Ming-Hong Tai - advisor
  • Files
  • etd-0805114-103028.pdf
  • Indicate in-campus at 99 year and off-campus access at 99 year.
    Date of Submission 2014-09-08

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