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博碩士論文 etd-0824105-132350 詳細資訊
Title page for etd-0824105-132350
南台灣原住民之血液生化指標與其飲酒習性及CYP2E1 5’側端區Rsa I/Pst I基因多型性之相關性研究
Association of the blood biochemical index with CYP2E1 5’flanking region Rsa I/Pst I gene polymorphism and alcohol consumption habit in Southern Taiwan aborigines
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Southern Taiwan aborigines, CYP2E1gene polymorphism
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CYP2E1屬於人體內酒精代謝途徑中微粒體乙醇氧化系統(MEOS)的酵素,在酒精代謝過程扮演重要角色,CYP2E1基因多型性影響個體對酒精的感受性,在基因5’ 側端區RsaI/PstI限制酶多型性,其突變型的c2對偶基因會增加基因轉錄活性、mRNA及蛋白質表現量。
酒精經CYP2E1轉變為乙醛過程中,產生大量活性氧,引發氧化壓力增加並且產生大量的自由基,引起細胞膜脂質過氧化作用,造成酒精性肝病與器官組織傷害,近年流行病學研究顯示,台灣地區原住民飲酒盛行率較非原住民高,Bosron 等人認為飲酒的行為受到環境與基因的影響,此種差異亦受族群影響甚大,本實驗藉由高飲酒率的原住民,探討飲酒習性與CYP2E1 Rsa I/Pst I基因多型性對血液生化指標之影響,實驗結果發現,飲酒的男性原住民在血壓、三酸甘油脂、高密度脂蛋白、尿酸及AST的數值皆高於未飲酒者,而在總膽固醇、低密度脂蛋白與肌酸酐的數值皆低於未飲酒者;而飲酒的女性原住民在血壓、高密度脂蛋白、尿酸、肌酸酐及AST的數值皆高於未飲酒者,而在總膽固醇、低密度脂蛋白的數值皆低於未飲酒者,但是飲酒的男性原住民及有無飲酒的女性原住民其CYP2E1基因型對生化指標之作用均未達到統計差異,未飲酒的男性原住民其CYP2E1基因型為c1/c2 + c2/c2與c1/c1相較,有較高的WHR (0.93±0.05 vs. 0.90±0.06,p = 0.035)及ALT (30.0±22.0 vs. 21.5±11.0,p = 0.012),
p值< 0.05具統計意義。本研究的結論為男性原住民CYP2E1基因型帶有c2對偶基因者,可能與肥胖及肝功能異常有關。
The microsomal ethanol oxidizing system (MEOS) is involved in metabolism of alcohol in the liver, the major component of MEOS is cytochrome P4502E1 (CYP2E1). CYP2E1 gene polymorphisms that alter its functions may be associated with alcohol susceptibility in individual. A RsaI/PstI restriction fragment length polymorphism (RFLP) has been reported in the 5’-flanking region of CYP2E1 gene. The rare mutant allele (c2 allele) that lacks the Rsa I restriction site, but can cut with Pst I has been found to be associated with higher transcriptional activity, mRNA expression, protein levels and enzyme activity than the commom wild type-c1 allele. CYP2E1-dependent oxidative stress on the pathogenesis of alcohol-induced liver injury. The recent epidemiological studies have point out that there are a high prevalence of alcohol consumption in aborigines of Southern taiwan. Dr.Bosron presented an drinks wine the behavior receives the environment and the genetic factor influence, this kind of difference also receives the racial influence to be really big. This study focus on the aborigines with high alcohol drinks rate to examine the possible effect on drinks habit and gene polymorphism of CYP2E1 RsaI/PstI to blood biochemicial index. The experimental result found that male aborigines drinker in blood pressure, triglyceride, HDL cholesterol, uric acid and AST value more than male aborigines non-drinker. However the values are in T.cholesterol, LDL cholesterol, creatinine less than non-drinker. The female aborigines drinker in blood pressure, HDL cholesterol, uric acid, creatinine and AST value more than female aborigines non-drinker. However the values are in T.cholesterol and LDL cholesterol less than non-drinker. Nevertheless, male aborigines drinker and female aborigines non-drinker did not achieved diversity of statistical in function of CYP2E1 gene polymorphism to biochemical index. Male aborigines non-drinkers have c1/c2+c2/c2 in CYP2E1 gene polymorphism compare to c1/c1 also have
higher WHR (0.93±0.05 vs. 0.90±0.06, p = 0.035) and ALT (30.0±22.0
IU/L vs. 21.5±11.0 IU/L , p = 0.012). To summary, male aborigines who have c2 allele in CYP2E1 gene polymorphism may relate to obese and Liver dysfuction.
目次 Table of Contents
目 錄

中文摘要 2
英文摘要 4
緒論 6
材料與方法 15
結果 24
討論 28
參考文獻 32
圖表 37
附錄 51
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樊台聖、李一靜、陳秀卿、趙善如、范慧華, 民國九十年, 原住民生活型態
與健康問題調查. 屏東科技大學學報第十卷第二期159-171頁.

柴國樑、郝立智, 2004年, 漫談脂肪肝. 台灣醫界第 47卷第一期13-17頁.
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