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|Type of Document
||Development of the interfaces for Coupling Ambient Ionization Mass Spectrometry with Gas and Liquid Chromatography|
|Date of Defense
||Ambient Ionization Mass Spectrometry
||The ion sources used in traditional gas chromatography mass spectrometry were always electron ionization or chemical ionization. However, the limitation of vacuum should be considered for these GC/MS systems.|
In this study, a new GC-ESI/MS interface was developed. This interface was constructed by running hot N2 gas through a metal tube in which the GC column was enclosed. The interface temperature was kept at 280 oC during analysis with a circumventing heating tape. The analytes eluted from GC and interface immediately joined an ESI plume which was located approximately 2 mm from the interface exit. The analyte ions were subsequently detected by a mass spectrometer. In addition, packed GC or capillary GC-ESI/MS analyses were examined under ambient conditions.
This GC-ESI/MS interface was used to detect derivatized amino acids in human urine. Amount of protonated analyte ions were detected due to the ESI ionization. The LOD could reach ppb level. In the analyses of engine oils, this interface was used for analysis of polar components in samples. In addition, principal component analysis (PCA) combined with GC-ESI/MS was examined for classify the quality of engine oils. This GC-ESI/MS interface coupled with pyrolysis, thermal desorption, Fast GC two dimensional GC were demonstrated.
Ion chromatography fused droplet electrospray ionization mass spectrometry was developed in this study. Because of the high salt tolerance, FD-ESI could coupled with ion chromatography to directly detect analyte ions.
||Maw-Rong Lee - chair|
Yeung-Haw Ho - co-chair
Hui-Fen Wu - co-chair
Shiuh-jen Jiang - co-chair
Jentaie Shiea - advisor
Indicate in-campus at 99 year and off-campus access at 99 year.|
|Date of Submission