Title page for etd-0911112-111354


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URN etd-0911112-111354
Author Hsin-yun Lin
Author's Email Address No Public.
Statistics This thesis had been viewed 5579 times. Download 2216 times.
Department Marine Biology
Year 2012
Semester 1
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Effects of dietary Bacillus subtilis spores on utilization of crystalline methionine in juvenile grouper, Epinephelus coioides, fed high plant-protein diets
Date of Defense 2012-08-17
Page Count 117
Keyword
  • Epinephelus coioides
  • soy protein
  • crystalline amino acid
  • digestibility
  • free amino acid
  • Abstract With the aim to enhance the efficiency of utilization of crystalline methionine supplemented in the high plant-protein diet for grouper (Epinephelus coioides), this study used Bacillus subtitlis spore as a probiotic additive in the diet to shorten the absorption time difference between protein-bound amino acid and crystalline methionine. The study was conducted in two parts. In the first part, juvenile groupers were fed for 14 weeks with 5 experimental diets: fish meal diet, high plant-protein diet with/without crystalline methionine, as well as with/without B. subtitlis spore separately. Growth performance, PER, protein digestibility, amino acid digestibility, non-specific immune responses, and free amino acid concentration in both muscle and serum were assessed. The second part was a time-series study on serum free amino acids concentration after a force-feeding experiment. The results showed that crystalline methionine supplementation in the high plant-protein diet effectively improved the growth of E. coioides (P<0.05). However, B. subtitlis spore supplementation did not affect fish growth performance significantly (P>0.05). A delay in the appearance of peak serum amino acid concentration was observed when fishmeal was partially replaced by soy protein. On the other hand, the force-feeding experiment showed that serum essential amino acid (include methionine) concentrations droped drastically after they reached the peak concentrations from being forced-fed with the B. subtilis containing diet. Supplementation of crystalline methionine seemed to ease the drop of serum methionine concentration. Based on these results, it is concluded that addition of B. subtitlis spore in high plant-protein diet for the grouper does not enhance the utilization of crystalline methionine, but supplementation of crystalline methionine significantly improve the growth performance of the grouper.
    Advisory Committee
  • Shi-Yen Shiau - chair
  • Hsieh-Pao Chuan - co-chair
  • Chen-Chih Kao - co-chair
  • Houng-Yung Chen - advisor
  • Files
  • etd-0911112-111354.pdf
  • Indicate in-campus at 0 year and off-campus access at 1 year.
    Date of Submission 2012-09-11

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