Title page for etd-0910112-163807


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URN etd-0910112-163807
Author Ya-Ting Yang
Author's Email Address silencemiffy@yahoo.com.tw
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Department Biological Sciences
Year 2011
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Burkholderia pseudomallei heat shock protein (groEL) DNA vaccination provides Th1 immune response with cross-protection to Burkholderia cenocepacia for BALB/c mice
Date of Defense 2012-07-30
Page Count 54
Keyword
  • Melioidosis
  • heat shock proteins
  • DNA vaccine
  • Burkholderia
  • Abstract The immunogenicity and protective efficacy of a DNA vaccine encoding a truncated groEL heat shock gene (pcDNA3/groEL) from Burkholderia pseudomallei was evaluated in vaccinated BALB/c mice infected with B. pseudomallei or B. cenocepacia. After vaccination, the levels of anti-GroEL total IgG and IgG2a were increased in mouse sera. The clonal expansion of the spleen cells increased, and the GroEL protein induced IFN-γ production by spleen cells. The anti-GroEL antibody-mediated opsonic killing effect was not able to eliminate the growth of B. pseudomallei but was able to eliminate the growth of B. cenocepacia. After intravenous challenge of the vaccinated Balb/c mice with B. pseudomallei, the number of bacteria colonizing the in liver and/or spleen was not reduced. Over 50% of vaccinated mice infected with B. pseudomallei died within 7 days post-infection. By contrast, the bacterial loads in organs were significantly reduced if the vaccinated mice were infected with B. cenocepacia. All of vaccinated mice were alive 7 days post-infection. Liver damage, as assessed by histological observation, and abnormalities in the levels of liver enzymes rapidly resolved in vaccinated mice. We suggest that B. pseudomallei groEL plasmid DNA immunization of Balb/c mice induces a Th1-type immune response and provides cross-protection against B. cenocepacia but not against B. pseudomallei infection.
    Advisory Committee
  • Jiin-Tsuey Chen - chair
  • Hsi-Hsun Lin - co-chair
  • Jong-Kang Liu - advisor
  • Ya-Lei Chen - advisor
  • Files
  • etd-0910112-163807.pdf
  • Indicate in-campus at 1 year and off-campus access at 1 year.
    Date of Submission 2012-09-10

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