Title page for etd-0910112-124755


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URN etd-0910112-124755
Author Ya-Ping Jhang
Author's Email Address No Public.
Statistics This thesis had been viewed 5386 times. Download 637 times.
Department Marine Biotechnology and Resources
Year 2011
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Isolation of Antibiotics Producing Soil Bacteria in Taiwan Intertidal Zones
Date of Defense 2012-07-24
Page Count 78
Keyword
  • antibiotics
  • marine bacteria
  • intertidal soils
  • secondary metabolites
  • Abstract Many bacterial diseases were controlled by antibiotics since mid 1900s.
    However, over use of the drugs leads to the prevalence of resistant strains, some of
    them are resistant to essentially all of the commercial antibiotics except for one or two.
    Therefore, new drugs are needed to combat the die-hard resistant pathogens. This
    study is aimed at isolating bacteria with antimicrobial activities from the intertidal
    soils of Taiwan. A total of 25 samples were collected from 5 locations, including
    Peng-Hu, Da-Peng Bay, Ken-ding, Gueishan Island and Little Liu-chiu. Marine Agar
    2216 and Actinomycete Isolation Agar were used for cultivation. Of 313 bacterial
    isolated, 47 of them showed antimicrobial activities against at least one of the 7
    indicators (Bacillus cereus, Staphylococcus aureus, Klebsiella pneumonia, Salmonella
    typhimurium, Pseudomonas aeruginosa, Vibrio harveyi, Escherichia coli). The 47
    strains were classified based on 16S rRNA phylogeny. The majority of them are
    Pseudoalteromonas spp. (31) and Bacillus spp. (9) as well as Virgibacillus sp. (1),
    Vibrio sp. (1), Streptomyces sp. (1) and Microbulbifer spp. (4). Over all, 10/47 are
    Gram positive and 37/47 require added salts for growth. The titers of antimicrobial
    substances as judged by ethyl acetate extraction were influenced by cultivation
    conditions, such as: growth temperature, types of media and time.
    Advisory Committee
  • Jong-Kang Liu - chair
  • Chih-Chuang Liaw - co-chair
  • Chi-Hsin Hsu - advisor
  • Files
  • etd-0910112-124755.pdf
  • Indicate in-campus at 5 year and off-campus access at 5 year.
    Date of Submission 2012-09-10

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