Title page for etd-0910112-122821


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URN etd-0910112-122821
Author Shyh-Yueh Puu
Author's Email Address No Public.
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Department Marine Biotechnology and Resources
Year 2012
Semester 1
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Chemical Constituents of the Formosan Soft Coral Nephthea chabrolii
Date of Defense 2012-08-23
Page Count 276
Keyword
  • secondary metabolites
  • Nephthea chabrolii
  • 19-norergosterols
  • anti-HCMV
  • cytotoxicity
  • Abstract Numerous bioactive secondary metabolites including sesquiterpenoids,diterpenoids, meroditerpenoids, and steroids have been isolated from the soft corals of the genus Nephthea. In order to search for novel bioactive substances from marine organisms, we have investigated the secondary metabolites of the organic extract of the soft coral Nephthea chabrolii collected at San-Hsian-Tai. Chromatographic fractionation of the acetone-soluble has led to the isolation of four 19-oxygenated steroids 1–4 and two 19-norergosterols 5, 6, along with twelve known compounds 7–18.
     The structures of these compounds were determined on the basis of their spectroscopic analysis data (1H NMR, 13C NMR, 1H–1H COSY, HSQC, HMBC, NOESY, IR, and HRESIMS), physical data and compared with the literature data. The cytotoxicity against of A-549 (human lung epithelial carcinoma), HT-29 (human colon adenocarcinoma), and P-388 (mouse lymphocytic leukemia) cells, and anti-HCMV (human cytomegalovirus) activity of compounds 1–6 were evaluated. Metabolites 1–6 displayed cytotoxicity against P-388 cell line with ED50 values of 0.93, 1.05, 1.20, 1.74, 1.19, 1.19 μg/mL. However, none of them exhibited inhibitory activity against HCMV (human cytomegalovirus).
    Advisory Committee
  • Jyh-Horng Sheu - chair
  • Chih-Chuang Liaw - co-chair
  • Fang-Rong Chang - co-chair
  • Ian-Lih Tsai - co-chair
  • Chang-Yih Duh - advisor
  • Files
  • etd-0910112-122821.pdf
  • Indicate in-campus at 99 year and off-campus access at 99 year.
    Date of Submission 2012-09-10

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