Title page for etd-0909111-114035


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URN etd-0909111-114035
Author Yu-Ting Liu
Author's Email Address No Public.
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Department Marine Biotechnology and Resources
Year 2010
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title The Surface Recognition on the VLPs of Dragon Grouper Nervous Necrosis Virus by its Antibodies
Date of Defense 2011-07-18
Page Count 93
Keyword
  • grouper nervous necrosis virus
  • monoclone antibody
  • virus-like particles
  • hybridoma
  • ascites
  • Abstract Grouper in Taiwan is of high value, but nervous necrosis virus infection causes
    100% mortality. Our laboratory had developed a good expression system to produce
    virus-like particles that induced immune functions. In this study, cells producing
    monoclonal antibody against the virus-like particles were used to induce BALB/c mice production of high titer ascites. The ascite from the H1 cells generated 6000-fold of antibodies higher than cell culture, using enzyme-linked immune-sorbent assay, although relatively less than the previous monoclonal cells of mX, 8000-fold. In the electron microscopy, the ascite antibody bound to various mutants of virus-like particles. Using the polymerase chain reaction to amplify fragments of IgG cDNAs, we will clone and express such cDNAs to efficiently produce the desired monoclonal antibodies.
    Advisory Committee
  • Chi-Hsin Hsu - chair
  • Wen Zhi-Hong - co-chair
  • Shu-Fen Chiou - co-chair
  • Tian-Lu Cheng - co-chair
  • Chan-Shing Lin - advisor
  • Files
  • etd-0909111-114035.pdf
  • Indicate in-campus at 99 year and off-campus access at 99 year.
    Date of Submission 2011-09-09

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