Title page for etd-0909108-102025


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URN etd-0909108-102025
Author Yung-ting Hsu
Author's Email Address No Public.
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Department Biological Sciences
Year 2007
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Clinicopathological Features in Oral Cavity
Squamous Cells Produced by podoplanin and Its
Functional Role in Head and Neck Cancer Cell Lines
Date of Defense 2008-07-29
Page Count 65
Keyword
  • podoplanin
  • oral squamous cell carcinoma (OSCC)
  • Abstract Head and neck cancer (HNC) makes up 6 % of the cancer patients
    in the world every year. This disease usually occurs in males and the
    incidence is increasing year by year. According to statistical analysis,
    HNC has less than 50 % five-year survival rate. Therefore, the research
    of HNC seems imminent so that may lead to the development of new
    approaches of diagnosis and therapy.
    Recent research had shown that expression of podoplanin caused
    cellular proliferation, and may be associated with tumor invasion,
    metastasis and malignant prognosis. Podoplanin, a mucin-type
    transmembrane sialoglycoprotein, is highly expressed in lymphatic
    endothelial cells but not expressed in vascular endothelial cells. The
    purpose of this study was to determine the clinical and pathological
    significance of podoplanin in oral squamous cell carcinoma (OSCC).
    Therefore, we collected clinical specimen and associated patient history
    of OSCC. Further, we used the human cell lines of HNSCC (Fadu, Hep2)
    to investigate the molecular regulation of podoplanin.
    Podoplanin expression was analyzed by RT-PCR and Western blot assay
    firstly. As shown, podoplanin was found to be overexpressed in tumors compared with normal adjacent tissues. Further, immunohistochemical
    analysis revealed the location of podoplanin expression in OSCC tissues.
    The results showed that podoplanin had higher expression in T4 stage
    tumor section than in normal adjacent tissues of OSCC samples, or in T1
    stage. Here, podoplanin was highly expressed in the OSCC tumor cell
    and lymphatics of stage T4 OSCC tissue. Furthermore, we found that
    overexpression of podoplanin in OSCC patients was associated with
    decreased five-years survival rate. In the univariate analysis, several
    factors were statistically significantly associated with disease-specific
    survival rate, including Tumor stage, Nodal stage, and podoplanin
    expression. In the subsequent multivariate analysis, only Tumor stage and
    Nodal stage showed a trend toward worse disease-specific survival. To
    further investigate the regulatory mechanism of podoplanin and its
    position of expression within the cell, immunofluorescence and
    transfection were utilized to assay. The results showed that podoplanin
    was expressed in the nuclear membrane of the Fadu and Hep2 cell lines,
    and the PI3K/AKT signaling pathway was involved. We suggest that the
    role of podoplanin in OSCC should be further investigated for potential
    future treatment.
    Advisory Committee
  • Cho, Chung-Lung - chair
  • Chien, Chin-Yen - co-chair
  • Chao, David - advisor
  • Files
  • etd-0909108-102025.pdf
  • indicate accessible in a year
    Date of Submission 2008-09-09

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