Title page for etd-0908109-170256


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URN etd-0908109-170256
Author Li-chuan Wu
Author's Email Address No Public.
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Department Biological Sciences
Year 2008
Semester 2
Degree Master
Type of Document
Language English
Title Hepatoprotective Effects of Pluchea indica (L.) Less. Aqueous Extract
against Thioacetamide-induced Liver Fibrosis in Mice
Date of Defense 2009-06-29
Page Count 70
Keyword
  • Hydroxyproline
  • Sirius red staining
  • Thioacetamide
  • Liver fibrosis
  • Pluchea indica
  • Abstract Typically chronic injury leads to hepatic fibrosis. No effective antifibrotic
    drugs have been approved, but herbal drugs have potential on the therapy of
    hepatic fibrosis. The objective of this study used TAA-induced liver fibrosis
    mouse as a model to elucidate whether aqueous extract of the root of Pluchea
    indica (PIAE) can reduce liver fibrosis triggered by TAA. Mice were
    intraperitoneally injected with TAA (200 mg/Kg) three times per week as the
    TAA group, and those of injected with PIAE once per week as the treatment
    group. Three PIAE dosages of low- (0.5 mg/ml), medium- (1.0 mg/ml), and
    high- (1.5 mg/ml) doses were applied. Control mice were intraperitoneally
    injected with phosphate-buffered saline (2 ml/Kg) three times per week. Mice
    were sacrificed after 4 or 8 week treatment. Mice serum glutamyl pyruvic
    transaminases (GPT) were increased in the TAA group while the treatment
    group effects were declined after 4 or 8 weeks. H&E, Reticular fiber, and Sirius
    red staining revealed that TAA induced liver fibrosis and fibrotic lesions were
    reduced by PIAE treatment. Hydroxyproline assay showed that TAA increased
    collagen contents and PIAE significantly decreased collagen contents after 4 or
    8 weeks. Collagen α1 and α-SMA mRNA levels were decreased after 4- or 8-
    week PIAE treatments. The protein levels of ED2, α-SMA, p53, and
    phospho-p53 were all significantly declined on 4 or 8 weeks after PIAE
    treatment. In conclusion, these results demonstrated that the aqueous extract of
    P. indica shows anti-fibrotic effects on fibrogenesis of mouse liver.
    Advisory Committee
  • Ching-Mei Hsu - chair
  • Li-Jen Liao - co-chair
  • Tse-Min Lee - co-chair
  • Chung-Lung Cho - advisor
  • Files
  • etd-0908109-170256.pdf
  • indicate in-campus access immediately and off_campus access in a year
    Date of Submission 2009-09-08

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