Title page for etd-0908107-120338


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URN etd-0908107-120338
Author Rong-fu Chen
Author's Email Address No Public.
Statistics This thesis had been viewed 5574 times. Download 3313 times.
Department Biological Sciences
Year 2006
Semester 2
Degree Ph.D.
Type of Document
Language zh-TW.Big5 Chinese
Title Immunopathogenesis of dengue-2 infection in a dengue-2 outbreak
Date of Defense 2007-06-22
Page Count 122
Keyword
  • dengue hemorrhagic fever
  • secondary infection
  • dengue fever
  • immunopathogenesis
  • cytokine
  • Abstract Incidence of dengue fever (DF) has been estimated a 30 fold increase in the past 50 years. Clinical manifestations of DF range from a simple febrile illness with physical soreness to life-threatening dengue hemorrhagic fever (DHF). The need for a better classification of the severity in DEN infections has been proposed to clarify the immunopathogenesis for the prevention and management of serious DEN infections. We attempted to investigate whether different mechanisms involved in the varied manifestations of bleeding tendency and vascular leakage in DF. In a hospital-based study, we first compared clinical features as well as laboratory data including virus load, T helper (Th1/Th2) cytokines, and vascular leakage-related mediators between patients with DHF and DF. Moreover, we defined another class of patients associated with bleeding tendency but not fulfilled with DHF criteria, called DF w/B, for a further comparison. The virus load in blood was not significantly different among DF, DHF and DF w/B. DF patients had a higher Th1 cytokine, IFNr, expression (70.0 ± 10.7 vs. 33.1 ± 8.0 vs. 33.0 ± 7.1 pg/ml; DF vs. DF w/B, p = 0.009; DF vs. DHF, p = 0.002), and both DHF and DF w/B patients had a significantly higher IL-10 levels (14.3 ± 4.1 vs. 26.2 ± 3.3 vs. 26.0 ± 3.5 pg/ml; DF vs. DF w/B, p = 0.023; DF vs. DHF, p = 0.016) than DF patients. Both DHF and DF w/B patients also had a higher rate of secondary dengue infection (DF w/B vs. DHF vs. DF: 50.0%, 74.4% and 14.3%, p < 0.001). By contrast, DHF but not DF w/B patients had significantly higher vascular leakage-related mediators: sVCAM-1, PGE2 and TNFα levels than DF patients. Patients with DF w/B had a higher platelet counts (DF w/B vs. DHF: 66.0 ± 8.3 vs. 20.7 ± 2.1 x109/L, p < 0.001) but lower ALT levels than those with DHF (DF w/B vs. DHF: 56.3 ± 7.7 and 144.7 ± 20.5 IU/L). This study provides new insight to different immune mechanisms involved in patients with DF, DF w/B, and DHF. DF involves augmented Th1 reaction, and DF w/B involves altered Th2 reaction, but DHF involves both altered Th2 reaction and augmented vascular insult. Clarification of the immune mechanisms among DF, DFw/B and DHF will facilitate certain specific treatment and prevention of DF patients from varied bleeding tendency and vascular leakage manifestations.
    Advisory Committee
  • Liu, Jong-Kang - chair
  • He, Shiping - co-chair
  • Hung, WC - co-chair
  • none - co-chair
  • Yang, Kuender D. - advisor
  • Cheng, Jiin-Tsuey - advisor
  • Files
  • etd-0908107-120338.pdf
  • indicate in-campus access immediately and off_campus access in a year
    Date of Submission 2007-09-08

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