| URN |
etd-0906107-172450 |
|---|
| Author |
Ju-Chien Lee |
|---|
| Author's Email Address |
ruchienkimo@yahoo.com.tw |
|---|
| Statistics |
This thesis had been viewed 5575 times. Download 2949 times. |
|---|
| Department |
Biological Sciences |
|---|
| Year |
2006 |
|---|
| Semester |
2 |
|---|
| Degree |
Ph.D. |
|---|
| Type of Document |
|
|---|
| Language |
zh-TW.Big5 Chinese |
|---|
| Title |
Ulva fasciata protein disulfide isomerase and thioredoxin expression in response to hypersaline stress |
|---|
| Date of Defense |
2007-07-16 |
|---|
| Page Count |
73 |
|---|
| Keyword |
Salinity stress
Oxidative stress
PDI
Polyamine
Ulva fasciata
|
|---|
| Abstract |
This research has investigated the gene expression of protein disulfide isomerase (PDI; EC 5.3.4.1) and thioredoxin (Trx) in the marine macroalga Ulva fasciata Delile in response to hypersaline (90‰) for 1 h. 90‰ induced H2O2 accumulation, reflecting the occurrence of oxidative stress. The contents of free and protein-bound SH were increased by 90‰. Trx transcripts increased in response to 90‰. PDI transcripts and enzyme activities increased in response to 90‰. H2O2 accumulation under 90‰ condition was increased by putrescine (Put) but decreased by spermidine (Spd) and spermine (Spm). By treatment of spermidine and spermine, the contents of free SH was increased and the contents of protein-bound SH decreased, showing that spermidine and spermine can increase free SH against oxidative stress. The gene expression and activity of PDI were further increased by Spd and Spm. Overall, the gene expression of PDI and Trx were responded to 90‰ for 1 hour and were adjusted protein–SH in polyamines treatment. |
|---|
| Advisory Committee |
Liu, Zin-Huang - chair
none - co-chair
Cho, Chung-Lung - advisor
Tse-Min Lee - advisor
|
|---|
| Files |
indicate access worldwide |
|---|
| Date of Submission |
2007-09-06 |
|---|
