Title page for etd-0904108-164845


[Back to Results | New Search]

URN etd-0904108-164845
Author Ting-ya Wang
Author's Email Address No Public.
Statistics This thesis had been viewed 5568 times. Download 1772 times.
Department Biological Sciences
Year 2007
Semester 2
Degree Master
Type of Document
Language English
Title Suppression of High Mobility Group Box-1 (HMGB-1) by RNAi Might Alter the Inflammatory Response During Sepsis
Date of Defense 2008-07-29
Page Count 46
Keyword
  • RNA interference (RNAi)
  • sepsis
  • cytokine
  • High mobility group box 1 (HMGB1)
  • Abstract High mobility group box 1 (HMGB-1) protein is a non-histone chromosomal protein. As a DNA binding protein, HMGB-1 is involved in the maintenance of nucleosome structure, regulation of gene transcription and it is active in DNA recombination and repair. It has been known that HMGB-1 is a late mediator of endotoxemia and sepsis. HMGB-1 is released from activated macrophages, induces the release of other proinflammatory mediators, and mediates cell death when overexpressed.
    We speculated that the course of sepsis maybe different without the involvement of HMGB-1. The aims of this study are to investigate the role of HMGB-1 in mediating sepsis and to observe the effects by using RNAi to affect the production of HMGB-1. Lipopolysaccharide (LPS) was used to simulate sepsis in culture as well as stimulate the release of HMGB-1 from RAW 264.7 cells. Levels of HMGB-1 in the culture medium were subsequently measured by Western blot. Other proinflammatory cytokines (TNF-α, IL-6 and TGF-β) were measured by ELISA. HMGB-1 could not be detected in the culture medium in the absence of LPS stimuli, but after 0.5 μg/ml LPS treatment HMGB-1 release could be detected. HMGB-1 the amount of released from LPS activated RAW 264.7 cells was in a time- and dose-dependent manner. The present study demonstrated that RNAi in the treatment of LPS-stimulated RAW264.7 cells resulted in the blockade of HMGB-1 and decreased LPS-induced inflammatory response. The results demonstrated that HMGB-1 plays a pivotal role in macrophage inflammatory responses by modulating the production of inflammatory mediators.
    Advisory Committee
  • Cho, Chung-Lung - chair
  • Lin, Meng-Chih - co-chair
  • Chao, David - advisor
  • Files
  • etd-0904108-164845.pdf
  • indicate accessible in a year
    Date of Submission 2008-09-04

    [Back to Results | New Search]


    Browse | Search All Available ETDs

    If you have more questions or technical problems, please contact eThesys