||This research investigate the matrix effect, operation time, cost and solvent comsumption for triarylmethanes and corresponding metabolites in different aquaculture products by different extraction methods. Triarylmethane was determined by LC-ESI-MS/MS in positive mode. The analytical method validation were followed the regulation of 2002/657/EC. HPLC separation was conducted with the RP-18 column. The mobile phases consisted of 0.5 mM ammonium acetate buffer (pH 3.8, adjusted with acetic acid) – acetonitrile (contained 0.1% formic acid) solution. |
The ratios of standard deviation to average residual time for all compounds are less than 0.05. The ion ratio is stable and allowed by the regulation of 2002/657/EC. The regression constant of calibration curve is in the range from 0.998 to 0.999. The CCα for MG, LMG, CV, LCV are 0.09 ± 0.05, 0.19 ± 0.14, 0.15 ± 0.13, 0.15 ± 0.10 μgkg-1, respectively. The CCβ for MG, LMG, CV, LCV are 0.09 ± 0.05, 0.19 ± 0.14, 0.15 ± 0.13, 0.15 ±0.10 μgkg-1, respectively.
The results for SPE and liquid-liquid extraction are not significantly different; the liquid-liquid extraction have shorter operation time, cheaper cost and less solvent consumption in comparison with SPE.