Title page for etd-0901110-165517


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URN etd-0901110-165517
Author Huang-Sung Hsiang
Author's Email Address No Public.
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Department Marine Biotechnology and Resources
Year 2009
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Comparison of The Solid Phase Extraction and The Liquid-Liquid Extraction Methods for Triarylmethanes and Corresponding Metabolites in Aquatic Animal Tissues
Date of Defense 2010-07-23
Page Count 94
Keyword
  • malachite green
  • crystal violet
  • triarylmethanes
  • Liquid-Liquid Extraction
  • Solid Phase Extraction
  • Abstract This research investigate the matrix effect, operation time, cost and solvent comsumption for triarylmethanes and corresponding metabolites in different aquaculture products by different extraction methods. Triarylmethane was determined by LC-ESI-MS/MS in positive mode. The analytical method validation were followed the regulation of 2002/657/EC. HPLC separation was conducted with the RP-18 column. The mobile phases consisted of 0.5 mM ammonium acetate buffer (pH 3.8, adjusted with acetic acid) – acetonitrile (contained 0.1% formic acid) solution. 
    The ratios of standard deviation to average residual time for all compounds are less than 0.05. The ion ratio is stable and allowed by the regulation of 2002/657/EC. The regression constant of calibration curve is in the range from 0.998 to 0.999. The CCα for MG, LMG, CV, LCV are 0.09 ± 0.05, 0.19 ± 0.14, 0.15 ± 0.13, 0.15 ± 0.10 μgkg-1, respectively. The CCβ for MG, LMG, CV, LCV are 0.09 ± 0.05, 0.19 ± 0.14, 0.15 ± 0.13, 0.15 ±0.10 μgkg-1, respectively.
    The results for SPE and liquid-liquid extraction are not significantly different; the liquid-liquid extraction have shorter operation time, cheaper cost and less solvent consumption in comparison with SPE.
    Advisory Committee
  • Chi-Hsin Hsu - chair
  • Jung-Hui Chen - co-chair
  • Wei-Hsien Wang - advisor
  • Files
  • etd-0901110-165517.pdf
  • indicate in-campus access immediately and off_campus access in a year
    Date of Submission 2010-09-01

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