Title page for etd-0901108-120157


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URN etd-0901108-120157
Author Ter-min Wang
Author's Email Address No Public.
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Department Marine Biotechnology and Resources
Year 2007
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Determination of the triarylmethanes and corresponding
metabolites in aquatic animal tissues by high-performance
liquid chromatography-tandem mass spectrometry
Date of Defense 2008-07-30
Page Count 131
Keyword
  • LC-MS/MS
  • high-performance liquid chromatography-tandem mass spectrometry
  • triarylmethane
  • Abstract There are two purposes in this research, one is the development of the new method which can be used for detection and quantification of triarylmethanes in fish tissues. The other is that we confirmed validation and utility of triarylmethanes by the method that is according to Commission Decision 2002/657/EC. Homogenized fish tissues were extracted twice with acetonitrile and defatted with n-hexane. HPLC separation was conducted with the RP-18 column. The mobile phases consisted of 0.5 mM ammonium acetate buffer (pH 3.8, adjusted with acetic acid)– ACN (contained 0.1% formic acid) solution. Triarylmethane was determined by LC-ESI-MS/MS in positive mode.
    The correlation coefficients of calibration curves with triarylmethane in fish tissues were 0.998 ~ 0.999. The decision limits (CCα) were 0.16 ± 0.07 μg/kg(MG), 0.15 ± 0.04 μg/kg(LMG), 0.20 ± 0.13 μg/kg(CV) and 0.23 ± 0.12 μg/kg(LCV), and detection capabilities (CCβ) were 0.20 ± 0.09 μg/kg(MG), 0.18 ± 0.05 μg/kg(LMG), 0.24 ± 0.16 μgkg-1(CV) and 0.29 ± 0.15 μg/kg(LCV).
    Advisory Committee
  • Chi-Hsin Hsu - chair
  • Jung-Hui Chen - co-chair
  • Wei-Hsien Wang - advisor
  • Files
  • etd-0901108-120157.pdf
  • indicate access worldwide
    Date of Submission 2008-09-01

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