| URN |
etd-0901108-120157 |
|---|
| Author |
Ter-min Wang |
|---|
| Author's Email Address |
No Public. |
|---|
| Statistics |
This thesis had been viewed 5575 times. Download 2253 times. |
|---|
| Department |
Marine Biotechnology and Resources |
|---|
| Year |
2007 |
|---|
| Semester |
2 |
|---|
| Degree |
Master |
|---|
| Type of Document |
|
|---|
| Language |
zh-TW.Big5 Chinese |
|---|
| Title |
Determination of the triarylmethanes and corresponding
metabolites in aquatic animal tissues by high-performance
liquid chromatography-tandem mass spectrometry |
|---|
| Date of Defense |
2008-07-30 |
|---|
| Page Count |
131 |
|---|
| Keyword |
LC-MS/MS
high-performance liquid chromatography-tandem mass spectrometry
triarylmethane
|
|---|
| Abstract |
There are two purposes in this research, one is the development of the new method which can be used for detection and quantification of triarylmethanes in fish tissues. The other is that we confirmed validation and utility of triarylmethanes by the method that is according to Commission Decision 2002/657/EC. Homogenized fish tissues were extracted twice with acetonitrile and defatted with n-hexane. HPLC separation was conducted with the RP-18 column. The mobile phases consisted of 0.5 mM ammonium acetate buffer (pH 3.8, adjusted with acetic acid)– ACN (contained 0.1% formic acid) solution. Triarylmethane was determined by LC-ESI-MS/MS in positive mode.
The correlation coefficients of calibration curves with triarylmethane in fish tissues were 0.998 ~ 0.999. The decision limits (CCα) were 0.16 ± 0.07 μg/kg(MG), 0.15 ± 0.04 μg/kg(LMG), 0.20 ± 0.13 μg/kg(CV) and 0.23 ± 0.12 μg/kg(LCV), and detection capabilities (CCβ) were 0.20 ± 0.09 μg/kg(MG), 0.18 ± 0.05 μg/kg(LMG), 0.24 ± 0.16 μgkg-1(CV) and 0.29 ± 0.15 μg/kg(LCV). |
|---|
| Advisory Committee |
Chi-Hsin Hsu - chair
Jung-Hui Chen - co-chair
Wei-Hsien Wang - advisor
|
|---|
| Files |
indicate access worldwide |
|---|
| Date of Submission |
2008-09-01 |
|---|
