Title page for etd-0824111-010108


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URN etd-0824111-010108
Author You-nan Lin
Author's Email Address No Public.
Statistics This thesis had been viewed 5336 times. Download 600 times.
Department Marine Biotechnology and Resources
Year 2010
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Determination and metabolism of ampicillin in tilapia by liquid chromatography-tandem mass spectrometry
Date of Defense 2011-07-22
Page Count 83
Keyword
  • LC/MS/MS
  • dispersive-solid-phase extraction
  • metabolism
  • ampicillin
  • talipia
  • Abstract In this study, a LC/MS/MS method for the determination of ampicillin antibiotic in fish muscle tissue was developed and accredited according to Commission Decision 2002/657/EC. The metabolism of ampicillin in tilapia was them studied in serum, liver and muscle.
      The homogenized fish tissue was first extracted with MeOH-H2O(4:1), C18 sorbent was added to remove lipids and impurities, the extract was then evaporated to dryness with a steam of nitrogen gas at 38 °C. The residue was redissolved with H2O, filtered and analyzed by LC/MS/MS equipped with an Agilient HC-C18(5μm, 150mm ×4.6mm), the mobile phase A was 10mM ammonium acetate containing 0.1% formic acid, while the mobile phase B was methanol. The determination of ampicillin was performed with electrospray ionization-tandem mass spectrometry in positive mode using multiple reation monitoring(MRM) for detection. Average recoveries were 81–86%, the limit of detection was 6.00 μg kg-1,decision limit(CCα) of ampicillin in fish muscle sample was 63.65 ± 7.99 μg kg-1.
      In the metabolism study, the oral administered dose to talipia was 20 mg/kg.BW. The maximum concentration of ampicillin in each tissues was obserned at 0.5 hour after oral administration, the maximum concentration in serum, liver and muscle was 27.53 mg L-1, 66.75 mg kg-1 and 1.33 mg kg-1, respectively. The concentration of ampicillin in muscle was 0.04 mg kg-1 24 hours after oral administration, which is lower than the 0.05 mg kg-1 MRL value of European Union resolutions. No residual ampicillin was detected in tilapia 48 hours after oral treatment, which conformed to the drug regulations for aquaculture ainmals in Taiwan.
    Advisory Committee
  • Chi-Hsin Hsu - chair
  • Jung-Hui Chen - co-chair
  • Wei-Hsien Wang - advisor
  • Files
  • etd-0824111-010108.pdf
  • Indicate in-campus at 5 year and off-campus access at 5 year.
    Date of Submission 2011-08-24

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