Title page for etd-0824110-131729


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URN etd-0824110-131729
Author Yi-Chen Wu
Author's Email Address janewu0922@gmail.com
Statistics This thesis had been viewed 5568 times. Download 2310 times.
Department Biological Sciences
Year 2009
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Bone Morphogenetic Protein-2 Plays an Antagonistic Role in Hepatic Fibrogenesis
Date of Defense 2010-07-16
Page Count 54
Keyword
  • BMP-2
  • Hepatic
  • fibrogenesis
  • TGF-β1
  • Abstract Hepatic injuries due to viral infection and alcoholic abuse frequently lead to activation and proliferation of hepatic stellate cells (HSCs), concomitantly with tissue repairing and remodeling mechanism. Transforming growth factor-β1 (TGF-β1) is known to be one of the potent cytokines that directly upregulates synthesis of extracellular matrix, thereby aggravating liver fibrosis. Bone morphogenetic protein-2 (BMP-2), as a member of TGF-Hepatic injuries due to viral infection and alcoholic abuse frequently lead to activation and proliferation of hepatic stellate cells (HSCs), concomitantly with tissue repairing and remodeling mechanism. Transforming growth factor-β1 (TGF-β1) is known to be one of the potent cytokines that directly upregulates synthesis of extracellular matrix, thereby aggravating liver fibrosis. Bone morphogenetic protein-2 (BMP-2), as a member of TGF-β1 superfamily, has been known to regulate cell proliferation, differentiation, and bone morphogenesis. Our previous study demonstrated that BMP-2 was downregulated in fibrotic liver of mice, supporting its antagonistic role in hepatic fibrogenesis. Downregulation of BMP-2 in fibrotic livers may lose its ability to antagonize the pro-fibrogenic action of TGF-β1. The purpose of this study was to determine whether mutual regulatory mechanisms exist between BMP-2 and TGF-β1.
    Treatment of recombinant protein, TGF-β1, significantly suppressed BMP-2 expression in hepatocytes clone-9 and HSC-T6 cells. Moreover, treatment of BMP-2 in both cell also attenuated TGF-β1protein levels in a dose-dependent manner. This finding supports that TGF-β1 and BMP-2 mutually modulated their expression not only in vivo, but also in vitro. Western blotting analysis showed that TGF-β1 and BMP-2 exerted different kinase phosphorylation profiles of signaling activities. However, the signal pathways and detail mechanisms of the interactions of BMP-2 and TGF-β1 in the fibrogenic action will need to further evaluate.
    Advisory Committee
  • Ching-mei Hsu - chair
  • hu-tsung Hui - co-chair
  • ming-hong Tai - advisor
  • Files
  • etd-0824110-131729.pdf
  • indicate in-campus access immediately and off_campus access in a year
    Date of Submission 2010-08-24

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