Title page for etd-0823107-174428


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URN etd-0823107-174428
Author Wei-han Pao
Author's Email Address hisashi9999.tw@yahoo.com.tw
Statistics This thesis had been viewed 5560 times. Download 4271 times.
Department Biological Sciences
Year 2006
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Analysis of the bacterial diversity in the Wu-Shan-Ding mud volcano areas
Date of Defense 2007-07-13
Page Count 107
Keyword
  • mud volcano
  • DGGE
  • Abstract This research is focus on the analysis of microbial diversity and existance of methanotrophic microbial strains in Wu-Shan-Ding mud volcano areas. The microbial distribution and diversity in soil surrounding the mud volcano were analyzed by polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE). The 16S rDNA sequences from PCR-DGGE bands were used to identify the bacterial strains with NCBI gene bank data. The results showed that the microbial diversity in the fresh erupted mud sample varied with other soil samples. The degrees of diversity were propotion to the distance away from the mud volcano. The main bacterial species found in the mud sample are Brevundimonas terrae, Ralstonia solanacearum, Ralstonia taiwanensis, Pseudonocardiaceae bacterium, Ochrobactrum anthropi, Burkholderia phytofirmans, Stenotrophmonas sp., Methylobacterium sp., Cryseobacterium sp., Sphingobacterium sp., Stenotrophomonas sp. MG-3, and Stenotrophomonas sp. EP01. According to documents, all of the above strains are tolerant to high salt and alkaline. The Ochrobactrum anthropi and Burkholderia phytofirmans were found only in the fresh erupted mud sample and the 5-meter soil sample, while Ralstonia mannitolilytica, Ralstonia pickettii, Amycolatopsis rugosa, and Maricaulis sp. were found only in 10-meter soil sample. In other word, all of the soil samples we examed in this study have their own specified bacterial strains. Two methanotrophic microganisms, Methylobacterium organophilum and Ochrobactrum anthropi were also successfully isolated in pure cultures. The biochemical characteristics of these two strains had been studied. These two strains show a potential might be able to use in the bioremediation of petroleum pollution.
    Advisory Committee
  • Jimmy Kao - chair
  • Jimmy Kuo - co-chair
  • Liu, Jong-Kang - advisor
  • Files
  • etd-0823107-174428.pdf
  • indicate in-campus access immediately and off_campus access in a year
    Date of Submission 2007-08-23

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