Title page for etd-0819108-111632


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URN etd-0819108-111632
Author Wen-jen Chen
Author's Email Address No Public.
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Department Biological Sciences
Year 2007
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title A proteomic analysis of Klebsiella oxytoca SYSU-011 after exposure to tetracyanonickelate(II)
Date of Defense 2008-06-13
Page Count 177
Keyword
  • 2-D PAGE
  • tetracyanonickelate
  • Abstract Klebsiella oxytoca SYSU-011 isolated from wastewater of a
    metal-plating plant in southern Taiwan had been proven to be able to
    degrade cyanide. In this study, we performed proteomic analyses to
    understand the mechanism of tetracyanonickelate (TCN) resistance in K.
    oxytoca by using two-dimensional polyacrylamide gel electrophoresis (2-D
    PAGE) and MALDI-TOF-MS techniques. There were 91 protein spots had
    been induced or overexpressed (≥2 fold) by TCN. Among them, 44 protein
    spots were successfully identified by MALDI-TOF-MS. The expressed
    proteins that had been escalated including chaperone, glutathione
    S-transferase (GST) and alkyl hydroperoxide reductase (AHR) were
    involved in the TCN detoxification process. Fructose-bisphosphate aldolase,
    glyceraldehyde-3-phosphate dehydrogenase, phosphoglucomutase and
    6-phosphogluconolactonase were involved in energy-producing process;
    nitrogenase and glutamine synthetase (GS) were required to regulate
    nitrogen assimilation. We also analyzed the K. oxytoca membrane proteins.
    Twenty six proteins spots had been successfully identified by
    MALDI-TOF-MS (out of 41 protein) that were induced ≥2 fold by TCN.
    These proteins induced RND-drive efflux proteins (RND family, MFP
    subunit, outer membrane factor A and outer membrane factor TolC) and cation efflux system. These efflux pumps could transport nickel ion out of
    the cells. The induced ATP-binding cassette (ABC) proteins may also play a
    role in transportation of metal-cyano complexes TCN and nutrition.
    By this study, we had a better understanding on the defense mechanism
    of K. oxytoca after exposure to TCN.
    Advisory Committee
  • Chih-Ming Kao - chair
  • Chan-Shing Lin - co-chair
  • Jong-Kang Liu - advisor
  • Ssu-Ching Chen - advisor
  • Files
  • etd-0819108-111632.pdf
  • indicate in-campus access in a year and off_campus not accessible
    Date of Submission 2008-08-19

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