Title page for etd-0816112-134849


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URN etd-0816112-134849
Author Ming-Chang Lin
Author's Email Address No Public.
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Department Marine Biotechnology and Resources
Year 2011
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Continued Study on the Secondary Metabolites and Bioactivities of the Soft Coral Klyxum molle
Date of Defense 2012-07-20
Page Count 229
Keyword
  • elastase
  • ROS
  • diterpenoids
  • soft coral
  • Abstract In order to search for bioactive compounds, we have studied the chemical constituents from the organic extracts of soft coral Klyxum molle. In this study, fifteen eunicellin-type diterpenoids, including eleven new compounds, klymollins K–U (1–11), along with four known compounds 12–15 were isolated. Compounds 16 and 17 were prepared by chemical synthesis. The structures of all compounds were established by spectroscopic methods and comparing the spectral data with known compounds. Compound 5 represents the first eunicellin-type compound with phenylacetyl group.
      The cytotoxicity of compounds 1–17 against K562 (human erythro myeloblastoid leukemia), Molt-4 (human acute lymphoblastic leukemia), and T47D (human breast earcinoma) were determined. Compounds 1, 2, and 3 exhibited weak cytotoxicity against Molt-4 (with ED50 11.52 ± 2.75, 20.41 ± 2.92, and 13.11 ± 3.87 μg/mL). Compound 5 was found to exhibt significant cytotoxicity toward K562, Molt-4, and T47D (with ED50 4.32 ± 1.38, 2.36 ± 0.34, and 4.65 ± 0.93 μg/mL). Compound 5 also displayed significant inhibitory effects on superoxide anion generation and elastase release by human neutrophils at 10 μg/mL (with Inh % 81.56 ± 3.23, and 89.16 ± 5.77).
    Advisory Committee
  • Chang-Yih Duh - chair
  • Chih-Chuang Liaw - co-chair
  • Fang-Rong Chang - co-chair
  • Jyh-Horng Sheu - advisor
  • Files
  • etd-0816112-134849.pdf
  • Indicate in-campus at 5 year and off-campus access at 5 year.
    Date of Submission 2012-08-16

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