Title page for etd-0815112-163439


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URN etd-0815112-163439
Author Yen-cheng Wu
Author's Email Address No Public.
Statistics This thesis had been viewed 5350 times. Download 198 times.
Department Electrical Engineering
Year 2011
Semester 2
Degree Master
Type of Document
Language English
Title A Neuron Emulator and Headstage Circuit for Patch Clamp Setups
Date of Defense 2012-07-30
Page Count 96
Keyword
  • patch clamp
  • neuron emulator
  • headstage
  • single electrode
  • action potential
  • current clamp
  • voltage clamp
  • Abstract This thesis presents a neuron emulator and headstage circuit for patch clamp setups and provides simulation, measurement and verification results. The circuit implemented on a printed circuit board (PCB) is battery powered and portable. The emulator provides both passive (resting potential) and active (action potential) electrical properties of a live neuron as seen from a single electrode by using the headstage circuit. It can be used to test electrophysiological equipment such as current-clamp, voltage-clamp or patch-clamp amplifiers. The action potentials (APs) are generated with a voltage-dependent frequency controlled by a microcontroller implementing a firing range from -60 mV to -30 mV and firing frequency from 1 Hz to10 Hz. The charge released by firing the neuron is initially stored on a 110 pC capacitor. Compared to directly using a current or voltage source, this design results in a more realistic simulation of the APs generated by ionic currents in a live neuron. The measured results from a prototype demonstrate that the neuron emulator meets the design specifications and it is capable of performing voltage clamp and rate responsive current clamp functionality. Measured results using a commercial clamp amplifier are provided to confirm the emulator operation in a practical recording environment.
    Advisory Committee
  • Jia-Jin Chen - chair
  • Jih-Ching Chiu - co-chair
  • Chung- Yao Kao - co-chair
  • Robert Rieger - advisor
  • Files
  • etd-0815112-163439.pdf
  • Indicate in-campus at 5 year and off-campus access at 5 year.
    Date of Submission 2012-08-15

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