Title page for etd-0815112-124200


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URN etd-0815112-124200
Author Fang-yu Chang
Author's Email Address No Public.
Statistics This thesis had been viewed 5565 times. Download 131 times.
Department Marine Biotechnology and Resources
Year 2011
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Continued Investigation on Chemical Constituents and Biological Activities of the Formosan Soft Coral Klyxum molle
Date of Defense 2012-07-18
Page Count 192
Keyword
  • cytotoxicity
  • anti-inflammatory
  • chromatography
  • eunicellin-type diterpenoids
  • Klyxum molle
  • Abstract In order to discover marine bioactive compounds, we investigated the chemical constituents from the organic extracts of soft coral Klyxum molle. Repeated column chromatography on the ethyl acetate extract of soft coral Klyxum molle, resulted in the isolation of nine new eunicellin-type diterpenoids, klymollins V-Z (1-5) and klyxumollins A-D (6-9), and four known compounds (10-13). The structures of new compounds were determined on the basis of extensive spectroscopic data (IR, MS, 1D and 2D NMR) and by comparison of the spectral data with those of the related known compounds.
    The cytotoxicity of compounds 1-13 was evaluated against five tumor cell lines including CCRF-CEM (human T cell lymphoblast-like cell line), K562 (human erythromyeloblastoid leukemia cell line), Molt 4 (human acute lymphoblastic leukemia cell line), T47D (human ductal breast epithelial tumor cell line) and DLD-1 (human colorectal adenocarcinoma cell line). Compound 2 exhibited weak cytotoxicity toward three cancer cells (CCRF-CEM, Molt 4 and T47D) and compounds 4 and 9 showed weak cytotoxicity against CCRF-CEM cancer cells. Compound 11 was found to exhibit stronger cytotoxicity toward CCRF-CEM cancer cells (ED50 values of 4.2 μg/mL). On the other hand, compound 3 showed weak activity against the accumulation of the pre-inflammatory cytokine IL-6 in LPS-induced macrophages, at a concentration of 25 μM.
    Advisory Committee
  • Chang-yih Du - chair
  • Chih-chuang Liaw - co-chair
  • Fang-rong Chang - co-chair
  • Jyh-horng Sheu - advisor
  • Files
  • etd-0815112-124200.pdf
  • Indicate in-campus at 5 year and off-campus access at 5 year.
    Date of Submission 2012-08-15

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