Title page for etd-0814112-153336


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URN etd-0814112-153336
Author Rong-Chang Jhong
Author's Email Address No Public.
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Department Institute of Biomedical Sciences
Year 2011
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Cytotoxicity and Proteomes Studies of AN30 on Oral Cancer Cell
Date of Defense 2012-07-25
Page Count 32
Keyword
  • oral cancer
  • proteomes
  • natural compound
  • Abstract The effect of natural compound AN30 on oral cancer cell line (Ca9-22), and normal oral cell line (HGF-1) was investigated. AN30 was purified from native fern plant Thelypteris torresiana. It had been reported to induce cell cycle arrest, apoptosis and DNA damage, cell growth inhibition in lung cancer cells, breast cancer cells and prostate cancer cells, but the effect in oral cancer is unknown. In a preliminary test, AN30 shows high toxicity to oral cancer cells but not normal oral cells. After treatment of AN30 to oral cancer cells, caspase activation and comet assays were performed to verity apoptosis induction. 2-D electrophoresis was used to find out the differentially expressed proteins between normal and cancer oral cell line under AN30 treatment. The proteins were further identified by LC/MS and western blot. The mechanism of these candidate proteins on drug toxicity remains further investigation.
    Advisory Committee
  • Hsueh-Wei Chang - chair
  • Kuang_Hung Cheng - co-chair
  • Hung-Wen Huang - advisor
  • Files
  • etd-0814112-153336.pdf
  • Indicate in-campus at 1 year and off-campus access at 99 year.
    Date of Submission 2012-08-14

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