Title page for etd-0812114-130535


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URN etd-0812114-130535
Author Hsiao-Yun Lai
Author's Email Address littlecloudylai@gmail.com
Statistics This thesis had been viewed 5386 times. Download 507 times.
Department Biological Sciences
Year 2013
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Analysis of the cytochrome oxidase system in Deinococcus radiodurans under different culture conditions
Date of Defense 2014-07-28
Page Count 113
Keyword
  • oxidizing enzyme activity
  • cytochrome c
  • TMPD
  • cytochrome constituent
  • Mn-CD effect
  • D. radiodurans
  • Abstract Deinococcus radiodurans is a highly radioresistant Gram-positive tetrad. Previously studies show that the addition of 2.5 μM of manganese (Ⅱ) into a stationary phase culture could trigger a new cycle of cell division (Mn-CD effect). In this study, we analyzed the cytochrome oxidase system in D. radiodurans under different culture conditions with the addition of manganese (Ⅱ) to further understand the pathway and efficiency of energy production. Results show that D. radiodurans grew well in all TY (per 100 ml containing 0.5 g tryptone and 0.3 g yeast extract), TGY (per 100 ml containing 0.5 g tryptone, 0.3 g yeast extract and 0.1 g D (+)-glucose), and TFY medium (per 100 ml containing 0.5 g tryptone, 0.3 g yeast extract and 0.1 g D-fructose). In which, the TFY medium provided the best nutrients for D. radiodurans to grow. The occurance of Mn-CD effect is related to the age of cells. The N, N, N ', N'-tetramethyl-p-phenylenediamine (TMPD), oxidase activities were found to be similiar in both TY and TGY grown cells. Both cells had the highest specific activity in the early log phase and the lowest specific activity in the stationary phase. However, the TFY medium grown cells were different, which performed the highest specific activity in the mid log phase. The specific activities of the Mn (Ⅱ)-free cells in all three media were found to be similiar, no matter in the early log phase, the mid log phase, and the stationary phase. For the cells with the addition of Mn (Ⅱ) from the beginning of cultivation, TY medium cells shew the highest specific activity in the early log phase, but decreased significantly in the stationary phase. For the cells with the addition of Mn (Ⅱ) to the eighteenth hour cultures, the TFY medium cells shew the highest specific activity in the mid log phase, but decreased significantly in the stationary phase. Therefore, the addition of Mn (Ⅱ) had a drastically effect to the TMPD specific activity during stationary phase. All D. radiodurans cells possessed cytochrome a1, a+a3, b, c, c+c1, and o, no matter cultured in the TY, TGY, or TFY medium. However, the content of each component, were various with different culture conditions. Results also show that the TMPD oxidase activity was not related to the amount of cytochrome c, which was different from previous literatures and need further study.
    Advisory Committee
  • Chi-Hsin Hsu - chair
  • Shu-Fen Chiou - co-chair
  • Jong-Kang Liu - advisor
  • Files
  • etd-0812114-130535.pdf
  • Indicate in-campus at 1 year and off-campus access at 1 year.
    Date of Submission 2014-09-12

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