Title page for etd-0812114-115102


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URN etd-0812114-115102
Author Yi-jen Lin
Author's Email Address No Public.
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Department Biological Sciences
Year 2013
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Evaluate the bioremediation of trinitrotoluene contaminated soil
Date of Defense 2014-07-28
Page Count 78
Keyword
  • High performance liquid chromatography (HPLC)
  • Denaturing gradient gel electrophoresis (DGGE)
  • 2
  • 4
  • 6-trinitrotoluene (TNT)
  • microcosm
  • bioremediation
  • Abstract The 2,4,6-trinitrotoluene (TNT) is a secondary explosive widely used in military and civil purposes all over the world. Residual TNT had been found in contaminated soil and groundwater mainly releasing from manufacturing or demilitarization facilities. Bioremediation of using microbial degradation is a very promising technique to treat environmental pollution problems. In this study, polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) was used to analyze the microbial communities of a TNT polluted site. Two TNT degrading bacteria, Achromobacter sp. and Klebsiella sp. were isolated from the soils that could use TNT as the sole nitrogen source for their growth. Aerobic microcosm studies were conducted using these two bacteria to assess the effectiveness of removal TNT from contaminated soil. Both basal salt medium (BSM) and nitrogen free glucose (NFG) medium mixed with TNT were added into the experimented microcosms. Results show that approximately 20% of TNT was removed in microcosms with BSM medium after 9 days of incubation. Complete TNT removal was observed in microcosms using NFG as the medium. Moreover, The TNT degradation by-products, 4-amino-2,6-dinitrotoluene (4-A-2,6-DNT) and 2-amino-4,6-dinitrotoluene (2-A-4,6-DNT) were detected in the microcosms.
    Advisory Committee
  • Chin-Ming Kao - chair
  • Chien-Cheng Chen - co-chair
  • Jong-Kang Liu - advisor
  • Files
  • etd-0812114-115102.pdf
  • Indicate in-campus at 99 year and off-campus access at 99 year.
    Date of Submission 2014-09-12

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