| Abstract |
In recent years, many marine natural products and their derivatives were found to have a variety of biological activities, especially for cancer drugs. Hepatocellular carcinoma (HCC) is one of the most common lethal cancers in the world, and become a heavy burden in many developing countries, including Taiwan. However, there are many side effects exist in current therapies of HCC. The extracts form soft coral Sinularia flexibilis were reported to have anti-cancer effects, and WY-12 is one of the compound origins from the soft coral. Hence, the aim of this study is to analyze whether WY-12 has the properties of anti-HCC and anti-angiogenesis. The present study shows that WY-12 could inhibit viability, migration, invasion, colony formation and anchorage-independent growth of the human HCC cell, SK-hep1. Western blotting and immunocytochemistry analysis indicated that WY-12 increased the level of pp38, HO-1 and E-cadherin, but decreased the expression of pERK and F-actin. Besides, WY-12 could also reduce viability and migration of the human umbilical vein endothelial cell line, EA.hy926, and suppress the expression of eNOS protein. The zebrafish data suggests that WY-12 has anti-angiogenesis effect. In conclusion, these results demonstrate that WY-12 seems to have multiple anticancer effects in HCC, and have the potential for anti-angiogenesis. |
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