Title page for etd-0805110-154227


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URN etd-0805110-154227
Author Ci-jie Lin
Author's Email Address m963020049@student.nsysu.edu.tw
Statistics This thesis had been viewed 5363 times. Download 1276 times.
Department Mechanical and Electro-Mechanical Engineering
Year 2009
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title DNA Molecules Stretching in Torus-type Microchannels
Date of Defense 2010-06-30
Page Count 114
Keyword
  • elongation flow and Relaxation time
  • hydrodynamic
  • CLSM
  • μPIV
  • DNA molecules
  • Abstract In this study, we design different inscribed/circumscribed circular torus-type microchannels to investigate the stretching behavior of DNA molecules. Strain rate and relaxation time play an important role in DNA stretching. In order to perform an analysis of the coil-stretch transition of DNA, we develop a method of stretching DNA molecules by using μPIV and CLSM measurements. μPIV is designed to measure the velocity distribution, after which the local strain rate can be estimated. The hydrodynamic stretching of DNA molecules in the elongation flow is observed using a confocal laser scanning microscope (CLSM). The relaxation time of the DNA molecules is then estimated according to the CLSM images analysis. At present, our experiments using the electro-osmotic flow (EOF) driven at various electric fields and viscosities to stretch DNA molecules show how one can investigate the influence of hydrodynamic interactions in the case of stretching of DNA molecules.
    Advisory Committee
  • Ching-Jenq Ho - chair
  • Chin-Chia Su - co-chair
  • Shou-Shing Hsieh - advisor
  • Files
  • etd-0805110-154227.pdf
  • indicate access worldwide
    Date of Submission 2010-08-05

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