Title page for etd-0804105-114954


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URN etd-0804105-114954
Author Chin-tan Chen
Author's Email Address m922050012@student.nsysu.edu.tw
Statistics This thesis had been viewed 5350 times. Download 1880 times.
Department Institute of Biomedical Sciences
Year 2004
Semester 2
Degree Master
Type of Document
Language English
Title Proteomic studies on development factors of pig embryonic stem cells into neural cells by RA in vitro
Date of Defense 2005-06-27
Page Count 81
Keyword
  • MALDI-TOF
  • vimentin
  • prohibitin
  • neural cells
  • ES cells
  • oct3/4
  • annexin A10
  • beta-catenin
  • neurod1
  • ZNF482
  • RA
  • neurogenin2
  • annexin A1
  • proteomic
  • sox2
  • FRK
  • Abstract Proteomic techniques were used to analyze the protein expression profile of the early-stage differentiation of pig embryonic stem cells (ES cells). The pig ES cells were induced to develop to neuronal cells by all-trans retinoic acid (ATRA) in vitro by Tainan Livestock Research Institute. The ES cells were cultured with ATRA and collected at time intervals of 0, 1, 2, 4, 8 and 10 days. The cell lysates were analyzed by two-dimensional electrophoresis, and the differentially expressed proteins are identified by MALDI-TOF. Our data shows that the expression profile of pig ES cells is similar to other mammalian models but with some differences. Preliminary pig ES cells 2D database was set up. Six spots each with up or down-regulation in neurogenesis were identified by MS. These proteins may become the good markers of pig ES cells into neural cells by RA. Among those proteins, vimentin, prohibitin and annexin A10 were up-regulated, zinc finger protein 482 (ZNF482), fyn-related kinase (FRK) and annexin A1 were down-regulated during differentiation of pig ES cells to neural cells. Addtionally, we ultilized RT-PCR technique to investigate mRNA expression during neurogenesis, vimentin and prohibitin was up-regulated, anxa1(annexin A1) was slightly down-regulated, neuroD1 and neurogenin 2 were high expression on day 10, beta-catenin was high expression on day 8 to 10.
    Advisory Committee
  • Yi-ren Hong - chair
  • none - co-chair
  • Hung-wen Huang - advisor
  • Files
  • etd-0804105-114954.pdf
  • indicate accessible in a year
    Date of Submission 2005-08-04

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