Title page for etd-0803116-090136


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URN etd-0803116-090136
Author Yu-chun Lin
Author's Email Address No Public.
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Department Department of Oceanography
Year 2015
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title The sponge assemblage and sponge-associated microbial composition at the seawater aquafarm in southern Taiwan
Date of Defense 2016-07-11
Page Count 81
Keyword
  • marine sponges
  • aquaculture
  • bacteria community
  • next generation sequencing
  • 16S rRNA
  • Abstract The aim of this study was to investigate the sponge species diversity and sponge-associated microbial composition at a seawater aquafarm in southern Taiwan. During the period of May 2015 - February 2016, six demosponges and one calcareous sponge species were found, including Clathrina aff. beckingae, Pachastrella aff. fusca, Hymeniacidon aff. flavia, Esperiopsis sp., Dragmacidon sp., Chalinula cf. amoyensisc and Suberites sp. All of them were Taiwan’s new records. Five of them grew steadily at sites of S2, S3, S4, and S7. Among the sites, S7 had much higher concentrations of phosphate, nitrate and nitrite than all others. Through the examination on the 16S rRNA V3~V5 region by Next Generation Sequencing (NGS) tool, six major bacterial phyla were identified, including Proteobacteria, Bacteroidetes, Firmicutes, Actinobacteria, Cyanobacteria, and Fusobacteria. Based on the generic level comparison, Pseudoalteromonas, Thalassomonas, Sediminicola and Vibrio were more abundant in seawater samples. In contrast, Azospirillum, Rickettsia, Nisaea and Acaryochloris had higher amount in sponge samples. The results indicated that sponges had their unique microbiome. Besides, different sponge species from the same sites had similar microbial compositions. However, the process of relation-establishment between sponges and sponge-associated microbials still needs further research.
    Advisory Committee
  • Kuo-Hsun Chiu - chair
  • Yusheng M. Huang - co-chair
  • Li-Lian Liu - advisor
  • Tsung-Ming Chen - advisor
  • Files
  • etd-0803116-090136.pdf
  • Indicate in-campus at 5 year and off-campus access at 5 year.
    Date of Submission 2016-09-03

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