Title page for etd-0801108-163719


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URN etd-0801108-163719
Author Shu-Jiun Huang
Author's Email Address No Public.
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Department Biological Sciences
Year 2007
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Effect of YDL100c Deficiency on the Growth of Saccharomyces cerevisiae in the Presence of Menadione
Date of Defense 2008-06-26
Page Count 48
Keyword
  • YDL100c
  • menadione
  • Abstract Wild type strain (WT) and YDL100c disrupted strain (KO) were grown at 30oC for 8 hr after adding 50 μM menadione. Cells of both strains were assayed for trehalose accumulation, intracellular molecular oxidation level, membrane lipid oxidation, and glutathione (GSH) content. The data showed both the molecular and membrane lipid oxidation levels are higher and the GSH content is lower in KO compared with WT in the presence of menadione. The catalase activity in KO strain are reduced than that in WT strain, catalase activity would affect in the presence of menadione. Further study of antioxidant gene expressions showed that TPS1 and CTT1 were involved in the general stress response;SOD1, GSH1, TRR1 and TRX2 were involved in the specific stress response. Above mentioned mRNA level were reduced, suggesting that the deletion of YDL100c in S. cerevisiae affects the operation of general and specific stress response when grown in the presence of menadione.
    Advisory Committee
  • Zin-Huang Liu - chair
  • Jong-Kang Liu - co-chair
  • Ching-Mei Hsu - advisor
  • Files
  • etd-0801108-163719.pdf
  • indicate in-campus access in a year and off_campus not accessible
    Date of Submission 2008-08-01

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