Title page for etd-0726114-095757


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URN etd-0726114-095757
Author Cian-rong Wu
Author's Email Address No Public.
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Department Biological Sciences
Year 2013
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title The relationship between C-reactive protein and hepatoma-derived growth factor in hepatoma carcinoma cell lines
Date of Defense 2014-07-30
Page Count 71
Keyword
  • HDGF
  • Liver cancer
  • nucleolin
  • CRP
  • CD64
  • Abstract Hepatocellular carcinoma (HCC) is one of the most common malignancy and known as country disease in Taiwan. Hepatoma-derived growth factor (HDGF), a mitogen for various types of cells, plays an important role in liver carcinogenesis. HDGF overexpression is associated with tumor progression and poor survival outcome in many types of cancer including lung cancer, melanoma and HCC. C-reactive protein (CRP) is a acute phase protein, belongs to a group of unique serum protein family and is mainly secreted by the liver. The serum CRP level rises rapidly by up to 1,000 times during acute or chronic inflammatory conditions such as infection and cardiovascular diseases. However, the role of CRP in liver carcinogenesis remains unclear. By using a panel of hepatoma cell lines, immunoblot analysis showed that CRP is highly expressed in all hepatoma cells regardless of their differentiation status. Exogenous CRP significantly stimulated the proliferation, wound healing, invasion and colonies formation of hepatoma cells. Besides, CRP increased CRP and its receptor CD64 expression in hepatoma cells. Interestingly, CRP also enhanced the expression of HDGF/nucleolin axis in hepatoma cells. Finally, CRP enhanced Akt phosphorylation and the expression of NFκB members. Because the role of HDGF in liver carcinogenesis and Akt/NFκB activation has been recently established, it seems plausible that CRP may mediate the malignant transformation of hepatoma cells through activation of HDGF/nucleolin axis.
    Advisory Committee
  • Li-Fen Liu - chair
  • Hoi-Hung Chan - co-chair
  • Ming-Hong Tai - advisor
  • Files
  • etd-0726114-095757.pdf
  • Indicate in-campus at 99 year and off-campus access at 99 year.
    Date of Submission 2014-08-30

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