Title page for etd-0726111-141617


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URN etd-0726111-141617
Author Fu-jui Tsao
Author's Email Address shiya0274@hotmail.com
Statistics This thesis had been viewed 5341 times. Download 3837 times.
Department Biological Sciences
Year 2010
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Bacterial diversity in the gastrointestinal tracts of four animals with different feeding habits
Date of Defense 2011-06-23
Page Count 91
Keyword
  • feeding habit
  • gastrointestinal tract microbiota ( GI tract microbiota )
  • bacterial diversity
  • denaturing gradient gel electrophoresis ( DGGE )
  • Abstract The animal phylogeny and feeding habits would affect the composition of gastrointestinal tract(GI tract)microbiota. GI tract microbiota plays an important role in host health and nutrient provision. In this study, we used PCR-DGGE and bacterial 16S rDNA sequencing to analyze the GI tract bacterial diversity of four animals with different feeding habits in Shou-Shan zoo, including one carnivore, one omnivore and two herbivores, in which one ruminant and one non-ruminant. The results show a great difference between GI tract bacterial diversity of the four animals. The abundance of GI tract bacterial diversity increased from carnivore, omnivore to herbivore. Comparing the similarity of the GI tract bacterial community structures of these four animals, the carnivore possessed the most different composition, to other animals, the next was the omnivore, while the two herbivores show the highest similarity to each other. Our results also indicated that the GI tract microbiota of these four different animals were very stable during the investigating period. We also found that two individuals of the same species had a very similar bacterial compositions in their GI tracts at different time point. This finding indicated that the bacterial compositions of GI tract in the four animals were affected mostly by the host phylogeny and their feeding habits. Moreover, according to bacterial 16S rDNA sequencing and idencification, results show that the Firmicutes were the dominant bacterial phyum in all four animals GI tracts, the amount of Bacteroides was much less than Firmicutes. This result might caused by the highly starch content in their feed. Large amount of carbohydrate-degrading, protein-degrading, lipid-degrading bacteria were found in all of these different animals. Fiber-degrading bacteria Fibrobacteres were identified in the GI tracts of the herbivores and omnivore, but not the carnivore, showing that GI tract microbiota plays an important role to provide nutrient and assist energy to the host.
    Advisory Committee
  • Chi-Hsin Hsu - chair
  • Jimmy C. M. Kao - co-chair
  • Jong-Kang Liu - advisor
  • Files
  • etd-0726111-141617.pdf
  • indicate accessible in a year
    Date of Submission 2011-07-26

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