Title page for etd-0725118-212646


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URN etd-0725118-212646
Author Ming-Hsu Cheng
Author's Email Address No Public.
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Department Mechanical and Electro-Mechanical Engineering
Year 2017
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title 3D Printed Cassette Embedded with Conductive Polymer Electrodes for Two-Dimensional Paper Chromatography and Paper Spray Mass Spectrometry (PS-MS) Detection in Bioanalysis and Food-safety Applications
Date of Defense 2018-07-23
Page Count 77
Keyword
  • Paper spray mass spectrometry
  • Paper-based microfluidic
  • Two-dimensional paper chromatography
  • 3D-printing
  • Abstract This paper presents a novel paper-based microfluidic cassette integrated two-dimensional paper chromatography and paper spray mass spectrometry (PS-MS) detection in bioanalysis and food-safety applications. The cassette can be fabricated in a single-process through a dual-extrusion 3D printer equipped with ABS and ABS conductive polymers such that delicate fabrication processes can be excluded for producing the cassette. The sample injection, separation, concentration and electrospray ionization could be completed in the single microfluidic cassette through the specially designed cassette, paper-based microfluidic and the embedded polymer electrode. The liquid sample is directly applied to the sample trench and then separated and concentrated using a 2D paper chromatography technique. To further improve the performance of the paper-based microfluidic system, several special designs including a tip contact for uniform developing solution dispersion, several sacrificial wings for sample smear elimination and sculpted holes for electrical isolation during paper spray ionization. Results shows that the sacrificial wing and throttle valve can increase the number of theoretical plate to about 3.9 and 1.6 times respectively in the first-dimensional chromatography, and greatly reduce the sample residue on the paper tip. The second-dimensional chromatography has increased the signal strength by nearly a hundred times. The developed system can detect the residual pesticides in fresh grape, and the residual drug in body fluid.
    Advisory Committee
  • Lung-Ming Fu - chair
  • Chan-Chiung Liu - co-chair
  • Wei-Lung Tseng - co-chair
  • Cheng-Hsin Chuang - co-chair
  • Che-Hsin Lin - advisor
  • Files
  • etd-0725118-212646.pdf
  • Indicate in-campus at 5 year and off-campus access at 5 year.
    Date of Submission 2018-08-25

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