| URN |
etd-0725114-131202 |
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| Author |
Ya-chun Chen |
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| Author's Email Address |
No Public. |
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| Statistics |
This thesis had been viewed 5573 times. Download 0 times. |
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| Department |
Institute of Biomedical Sciences |
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| Year |
2013 |
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| Semester |
2 |
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| Degree |
Master |
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| Type of Document |
|
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| Language |
English |
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| Title |
Studies on the relationship between autophagy and apoptosis in hepatocellular carcinoma-derived cells Huh7 by treatment with ABT-751 |
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| Date of Defense |
2014-07-28 |
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| Page Count |
53 |
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| Keyword |
Hepatocellular carcinoma
Autophagy
Apoptosis
Microtubule
ABT-751
|
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| Abstract |
The objective was to study the effects of ABT-751 on apoptosis and autophagy in hepatocellular carcinoma-derived cells. Hepatocellular carcinoma (HCC) is a common malignant tumor in the world. In recent years, HCC was the second leading cause of cancer death in Taiwan. Recently, the effects of anti-cancer drugs usually are mediated by the inhibition of tumor angiogenesis and microtubule synthesis. ABT-751 arrest cells in the G2/M phase due to its inhibition of microtubule synthesis and induction of cell apoptosis. The objective of thesis is to study the relationship between autophagy and apoptosis in hepatocellular carcinoma-derived cells Huh7 by treatment with ABT-751.
In this study, the cytotoxicity of ABT-751 to Huh7 cells was measured by MTT assay at 570 nm. The results indicated the IC50 of Huh7 treated with ABT-751 for 48 h was 1.5 µM. The assay of Cyto-ID, acridine orange staining and immunoblotting were used to determine the ABT-751-induced autophagy in Huh7 cells and the autophagy induced by ABT-751 has a role in cell survival. To investigate whether inhibition of autophagy could enhance apoptosis in Huh7 cells after the treatment of ABT-751. The results showed the inhibition of autophagy could enhance the effect of inducing Huh7 cells death due to the combinations of ABT-751 with the autophagy inhibitors 3-methylamphetamine (3-MA) or bafilomycin A1 (BafA1). Above results indicated that ABT-751 could inhibit microtubule polymerization and lead to autophagy, which promoted survival of cancer cells. Consequently, combinations of ABT-751 with the autophagy inhibitor, which will promote the effect of ABT-751 to induce cytotoxin. |
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| Advisory Committee |
Yao-tsung Yeh - chair
Hung-Wen Huamg - co-chair
Yow-Ling Shiue - advisor
|
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| Files |
Indicate in-campus at 99 year and off-campus access at 99 year. |
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| Date of Submission |
2014-08-25 |
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