Title page for etd-0725114-131202


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URN etd-0725114-131202
Author Ya-chun Chen
Author's Email Address No Public.
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Department Institute of Biomedical Sciences
Year 2013
Semester 2
Degree Master
Type of Document
Language English
Title Studies on the relationship between autophagy and apoptosis in hepatocellular carcinoma-derived cells Huh7 by treatment with ABT-751
Date of Defense 2014-07-28
Page Count 53
Keyword
  • Hepatocellular carcinoma
  • Autophagy
  • Apoptosis
  • Microtubule
  • ABT-751
  • Abstract The objective was to study the effects of ABT-751 on apoptosis and autophagy in hepatocellular carcinoma-derived cells. Hepatocellular carcinoma (HCC) is a common malignant tumor in the world. In recent years, HCC was the second leading cause of cancer death in Taiwan. Recently, the effects of anti-cancer drugs usually are mediated by the inhibition of tumor angiogenesis and microtubule synthesis. ABT-751 arrest cells in the G2/M phase due to its inhibition of microtubule synthesis and induction of cell apoptosis. The objective of thesis is to study the relationship between autophagy and apoptosis in hepatocellular carcinoma-derived cells Huh7 by treatment with ABT-751.
    In this study, the cytotoxicity of ABT-751 to Huh7 cells was measured by MTT assay at 570 nm. The results indicated the IC50 of Huh7 treated with ABT-751 for 48 h was 1.5 ┬ÁM. The assay of Cyto-ID, acridine orange staining and immunoblotting were used to determine the ABT-751-induced autophagy in Huh7 cells and the autophagy induced by ABT-751 has a role in cell survival. To investigate whether inhibition of autophagy could enhance apoptosis in Huh7 cells after the treatment of ABT-751. The results showed the inhibition of autophagy could enhance the effect of inducing Huh7 cells death due to the combinations of ABT-751 with the autophagy inhibitors 3-methylamphetamine (3-MA) or bafilomycin A1 (BafA1). Above results indicated that ABT-751 could inhibit microtubule polymerization and lead to autophagy, which promoted survival of cancer cells. Consequently, combinations of ABT-751 with the autophagy inhibitor, which will promote the effect of ABT-751 to induce cytotoxin.
    Advisory Committee
  • Yao-tsung Yeh - chair
  • Hung-Wen Huamg - co-chair
  • Yow-Ling Shiue - advisor
  • Files
  • etd-0725114-131202.pdf
  • Indicate in-campus at 99 year and off-campus access at 99 year.
    Date of Submission 2014-08-25

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