Title page for etd-0725111-142515


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URN etd-0725111-142515
Author Shu-Chiu Shie
Author's Email Address No Public.
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Department Marine Biology
Year 2010
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Photosynthetic electron transport modulates genes expression of Methionine Sulfoxide Reductase (MSR) in Chlamydomonas reinhardtii
Date of Defense 2011-07-08
Page Count 91
Keyword
  • methionine sulfoxide reductase
  • high light
  • photosynthetic electron transport (PET)
  • Chlamydomonas reinhardtii
  • DBMIB
  • DCMU
  • QA
  • PQ
  • mixotrophic culture
  • autotrophic culture
  • Abstract Chlamydomonas reinhardtii can utilize CO2 for autotrophic growth (HSM plus
    5% CO2) or acetate for mixotrophic growth (TAP). This study was to elucidate the
    differential regulation of methionine sulfoxide reductase (MSR) gene expression
    between HSM plus 5% CO2 and TAP cultured cells, and also to determine the
    difference of gene expression in response to high light (1,000 μE m-2 s-1). The role of
    photosynthetic electron transport (PET) in the regulation of MSR gene expression was
    also examined by the use of PET inhibitors. High light inhibited PSII activity (Fv/Fm
    and Fv'/Fm') of HSM plus 5% CO2 and TAP cultured cells., while the responses of
    CrMSR gene expression in mixotrophically grown cells were different from
    autotrophically grown cells, High light increased the expression of CrMSRA1,
    CrMSRA2, CrMSRA3, CrMSRA5, CrMSRB1.2, and CrMSRB2.1, but inhibited the
    expression of CrMSRA4 and CrMSRB2.2 in autotrophically grown cells. The
    expression of CrMSRA3, CrMSRA5, and CrMSRB2.1 in mixotrophically grown cells
    was increased by high light but that of CrMSRA1, CrMSRA4, and CrMSRB2.2 was
    inhbited. The number of MSR isoform that was up-regulated by high light was greater
    in autotrophically grown cell than that in mixotrophically grown cells. Using the PET
    inhibitors (3-(3,4-dichlorophenyl)-1,1- dimethylurea (DCMU) and
    2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB)), most of the CrMSRA
    expression was regulated by reduced QA for autotrophically grown cells while
    reduced PQ was the main site for mixotrophically grown cells by high light. The
    expression of CrMSRB in autotrophically grown cells was mainy modulated by QA (-)
    or Cytb6f (-), while that was not affected by PET, except a role of Cytb6f (-) on the
    high light-induced CrMSRB2.2 expression. We fouind that CrMSRB gene expression
    in autotrophically grown cells was highly affected by PET but not for micotrophically
    grtown cells. The present result that H2O2 did not accumulate in autotrophically and
    mixotrophically grown cells suggests that H2O2 may be not involved in the regulation
    of high light regulation of CrMSR gene expression. The present study shows that the
    mRNA expression of CrMSR isoforms in Chlamydomonas was diffrerentially
    regulated between autotrophically and mixttrophically grown cells. The relationship
    between the utilization of different C source and CrMSR gene expression will be
    discussed.
    Advisory Committee
  • Hsien-Jung Chen - chair
  • Yi Ting Hsu - co-chair
  • Tse-Min Lee - advisor
  • Files
  • etd-0725111-142515.pdf
  • indicate in-campus access in a year and off_campus not accessible
    Date of Submission 2011-07-25

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