Title page for etd-0724116-004046


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URN etd-0724116-004046
Author Che-wei Lin
Author's Email Address No Public.
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Department Mechanical and Electro-Mechanical Engineering
Year 2015
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Novel Thread-based Capillary Electrophoresis Electrochemical System integrated Electrospray Ionization for Mass Spectrometry Detection in Bioanalysis and Food-safety Applications
Date of Defense 2016-07-12
Page Count 90
Keyword
  • Thread-based microfluidic
  • Sample pinch focus
  • Capillary electrophoresis separation
  • Electrochemical detection
  • Electrospray ionization
  • Abstract This research presents the first microfluidic chip for integration of capillary electrophoresis electrochemical (CE-EC) detection and electrospray ionization (ESI). It can be used for rapid bio and food sample analysis. Polyester thread of 200 ┬Ám in diameter is used as the liquid route such that delicate channel fabrication process can be excluded. The entire detection process of this system can be done by only a straight polyester thread channel, which break the conventional concept of sample manipulation. This new design also bring us some benefits, such as liquid sample can be directly applied at arbitrary site of the thread for electrophoresis separation. In order to improve the efficiency of single thread based CE separation, two buffer drops are applied at neighboring side of the sample plug to shorten it, which is called in-line sample pinch focus process. Image results shows that the length of sample plug can be reduced to about 38% of its original after the focus process. In addition, the current response of EC detection shows 2.7 times enhancement, and the theoretical plate number also increase 2.8 times. A simple fixture structure is designed to hold the thread, and the tail end of thread is then applied a high voltage for ESI. The thread-based ESI can easy be stabilized for more than 30 min with a high ion intensity of 109 ions/cm3. The ingredients of commercial eye drops and energy drink-Red Bull are detected with high signal to noise ratio of more than 10. Finally, the combination of CE-EC detection and ESI is achieved by the developed single thread microfluidic. Bio samples of dopamine and ascorbic acid, and food sample of Supau are both successfully be detected by these two methods. The combination of two detection methods can let them complement each other's shortage, which shows great potential for bio and food applications.
    Advisory Committee
  • Lung-Ming Fu - chair
  • Chien-Chou Tseng - co-chair
  • Shin-Pon Ju - co-chair
  • Wen-Cheng Kuo - co-chair
  • Che-Hsin Lin - advisor
  • Files
  • etd-0724116-004046.pdf
  • Indicate in-campus at 5 year and off-campus access at 5 year.
    Date of Submission 2016-08-25

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