Title page for etd-0723109-084810


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URN etd-0723109-084810
Author Cheng-feng Tsai
Author's Email Address No Public.
Statistics This thesis had been viewed 5364 times. Download 1836 times.
Department Mechanical and Electro-Mechanical Engineering
Year 2008
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Stretching and Deformation of DNA Molecules in a Converging-Diverging Microchannel with Heating Effect
Date of Defense 2009-07-02
Page Count 97
Keyword
  • thermal effects
  • μPIV
  • conformation and dynamics
  • DNA molecule
  • CLSM
  • Abstract In this study, an electrokinetics-induced elongation flow was created inside a gradual converging-diverging microchannel with different temperature (25, 35, 45, 55°C). The conformation of DNA molecules, local strain rate, and the relaxation time play important roles in determining the extent of DNA stretching. By using μPIV/μLIF measurements, the velocity/temperature distributions in microchannels can be secured. The local strain rate was estimated by μPIV measurements. We observe the hydrodynamic stretching DNA molecules in elongation flow by confocal laser scanning microscope (CLSM). Through CLSM images analysis, relaxation time of DNA molecules was estimated. Finally, dynamic properties and stretching ratio of DNA molecules stretched by EOF driven at various electric field and temperature ware measured. The thermal effect and the electric field on the conformation were also studied and discussed.
    Advisory Committee
  • Ching-Jeng Ho - chair
  • Chin-Chia Su - co-chair
  • Shou-Shing Hsieh - advisor
  • Files
  • etd-0723109-084810.pdf
  • indicate access worldwide
    Date of Submission 2009-07-23

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