Title page for etd-0721115-164606


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URN etd-0721115-164606
Author Chun-Sheng Chang
Author's Email Address No Public.
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Department Biological Sciences
Year 2014
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Bioremediation of trichloroethylene contaminated sites enhanced by emulsified carbon-releasing substrate
Date of Defense 2015-07-20
Page Count 102
Keyword
  • hydA gene
  • denaturing gradient gel electrophoresis
  • real-time PCR
  • emulsified carbon-releasing substrate
  • trichloroethylene
  • Abstract The emulsified carbon-releasing substrate (ECS) is commonly used in bioremediation that can promote the microbial growth and enhance biodegradation process. The remediation object of this study is a local trichloroethylene (TCE) contaminated site. Based on the direction of the groundwater flow, there are five deep wells were dug in the remediation site, i.e. P64, P48, TW11, P22, and P24 respectively. P22 is the injection well and the others are sampling and monitoring wells. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) technique was used to monitor the microbial diversity during the remediation process. The real-time PCR was also used to detect the quantity of Dehalococcoides species as well as 3 dehalogenase genes (TceA、VcrA、BvcA) to evaluate the dechlorination activity. Results show that well P22 contained the most microbial diversity than all other wells because the injected ECS encouraged the microbial growth. The qPCR studies revealed that the content of Dehalococcoides species was related to pH value, the lower the pH value the less Dehalococcoides species present. However, the pH value did not overly affect the growth of Dehalococcoides species. The oxidation-reduction potential(ORP) indicated that the contaminated site was anaerobical and could stimulate the reductive dechlorination process. A hydrogen production Clostridium butyricum bacterial strain was isolated from the groundwater. The hydrogen produced by this strain can be used as an electron donor for TCE dechlorination. The expression of hydA gene of the anaerobic batch culture reached the highest amount (ie. 1.7× 1011 gene copies / L) at 28 day. The steady decreasing of TCE concentrations suggested that C. butyricum could provide hydrogen continually to assist the anaerobic dechlorination reaction.
    Advisory Committee
  • Jimmy C.M. Kao - chair
  • Lei Yang - co-chair
  • Jong-Kang Liu - advisor
  • Files
  • etd-0721115-164606.pdf
  • Indicate in-campus at 99 year and off-campus access at 99 year.
    Date of Submission 2015-08-21

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