Title page for etd-0715118-104225


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URN etd-0715118-104225
Author Chih-Yen Tung
Author's Email Address No Public.
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Department Department of Oceanography
Year 2017
Semester 2
Degree Master
Type of Document
Language English
Title Population connectivity of Terapon jarbua (Pisces: Perciformes) in the South China Sea
Date of Defense 2017-07-27
Page Count 63
Keyword
  • cytochrome b
  • COI
  • population community
  • the South China Sea
  • Terapon jarbua
  • Abstract Most studies on the genetic structure of fishes in the South China Sea (SCS) showed weak genetic structure probably due to long pelagic larval duration (PLD) and long-distance migration of large size species. In this study, Terapon jarbua, a small size fish with a shorter PLD, was selected as our target species, collected from 12 sampling sites along the SCS, including Kaohsiung, Dongsha Atoll, Taiping Island, Raoping, Hong Kong, Hai Phong and Ninh Thuan of Vietnam, Sihanoukville of Cambodia, Kuantan and Kuching of Malaysia and Cebu and Alaminos of the Philippines. In this research, population connectivity of T. jarbua is studied in order to evaluate the influence of currents on its population genetic structure. Fragments of cyt b and COI (1752 bps) of 180 specimens of T. jarbua were sequenced to study their relationships among populations. Maximum likelihood tree and median-joining network showed that all haplotypes were divided into two lineages (A and B), and lineage A was further divided into two sublineages (1 and 2). Among the divisions, lineage B and sublineage 1 comprised individuals exclusively from Kuching while sublineage 2 contained individuals from all sampling sites. Interspecific genetic distance based on the comparison to T. theraps suggests that cryptic species may not be an issue in these two lineages. In addition to our own data, COI sequences from previous study were downloaded from GenBank to reconstruct the network. The median-joining network is divided into four groups, in which sublineage 2 of the present study fell into Pacific group of previous study, and sublineage 1 was identical to their Indian group. On the other hand, no haplotype of Iles Eparses group is collected in the SCS and lineage B that is exclusively from Kuching is not present in the previous study. FST values and Barrier analysis showed that gene flows between Malaysian water and other sites were at least partially limited, and a strong genetic barrier separated Kuching from all other locations while a weaker barrier located in the middle of the SCS. Based on the results from Barrier, AMOVA analysis showed that the genetic structure of Kuching was significant. Tajima’s D, Fu’s Fs, mismatch distribution, Goodness-of-fit test, demographic expansion parameters and Bayesian Skyline plot all suggested that lineage A experience a demographic expansion (0.04~0.01 Ma), and the expansion of lineage B would be much smaller or lineage B has undergone a stable growth. According to maximum credibility tree, most of the divergence time for T. jarbua corresponded to the middle to late Pleistocene.
    Advisory Committee
  • Jen-Chieh Shiao - chair
  • Min-Yun Liu - co-chair
  • Te-Yu Liao - advisor
  • Files
  • etd-0715118-104225.pdf
  • Indicate in-campus at 2 year and off-campus access at 2 year.
    Date of Submission 2018-08-15

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