Title page for etd-0714117-121755


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URN etd-0714117-121755
Author Tsen-hung Lin
Author's Email Address No Public.
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Department Marine Biotechnology and Resources
Year 2016
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Glutathione Regulation of the Acclimation of Chlamydomonas reinhardtii to High-intensity Illumination: the Importance of Glutathione Redox Status
Date of Defense 2017-05-31
Page Count 118
Keyword
  • high light stress
  • Chlamydomonas reinhardtii
  • Glutathione Reductase (GR)
  • Glutathione
  • tolerance
  • oxidative stress
  • Abstract This study examines the role of glutathione reductase (GR, EC 1.6.4.2) in the tolerance of a green alga Chlamydomonas reinhardtii (Chlamydomonas reinhardti PA Dangeard, C. reinhardtii) to light stress. We found that C. reinhardtii showed a decrease in photosynthetic rate and glutathione (GSH) content within 3 hours after high light (HL; 1,400 μE m-2 s-1) and recovered after 9-24 hours. Upon exposure to HL stress, glutathione reductase gene expression and enzyme activity increased, but glutathione redox ratio (GSH/GSSG) decreased. It is proposed that the increase in GSH and the maintenance of GSH/GSSG ratio through enhanced GR activity are related to the tolerance of C. reinhardtii to HL stress. This study examines the role of GR in HL stress tolerance in C. reinhardtii via downregulation and overexpression were performed on C. reinhardtii glutathione reductase 1 (CrGSHR1; NCBI number: XM_001696527.1; JGI number : Cre06.g262100) and glutathione reductase 2 (CrGSHR2; NCBI number : XM_001694648.1; JGI number: Cre09.g396252). We have obtained two CrGSHR1-knockdown lines (GSHR1-ami 102 and 105) and CrGSHR1-overexpressing line (GSHR1-77) and CrGSHR2-overexpressing line (GSHR2-136). The CrGSHR1-knockdown line was in tolerant to 1,400 μE m-2 s-1 illuminatine. Overexpression line can increase their survival and low lipid peroxidation after treatment with high-intensity illumination (1,800 μE m-2 s-1). Taken together, GSH conversion efficiency can be improved by CrGSHR and reduce oxidative damage while increasing the resistance of C. reinhardtii to high light stress.
    Advisory Committee
  • Kuo-Chen Yeh - chair
  • Su-Chiung Fang - co-chair
  • Chwan-Yang Hong - co-chair
  • Hsien-Jung Chen - co-chair
  • Tse-Min Lee - advisor
  • Files
  • etd-0714117-121755.pdf
  • Indicate in-campus at 5 year and off-campus access at 5 year.
    Date of Submission 2017-08-14

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