Title page for etd-0714108-114551


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URN etd-0714108-114551
Author Shu-Hsun Cheng
Author's Email Address No Public.
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Department Biological Sciences
Year 2007
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title The bacterial diversity in a KaoPing River constructed wetland for wastewater treatment
Date of Defense 2008-06-13
Page Count 158
Keyword
  • denaturing gradient gel electrophoresis (DGGE)
  • microbial diversity
  • KaoPing River Rail Bridge constructed wetland
  • Abstract    Constructed wetlands had been used for water treatment worldwide. The efficiency of wastewater treatment in a constructed wetland depends on its design, types of aquatic plants and microbial community present in this wetland. The goal of this study is to analyze the microbial populations in KaoPing River Rail Bridge constructed wetland which was designed to remove the polluted material from municipal sewage and industrial wastewater. Sediment and water samples were collected every 3 months from April, 2007 to April, 2008. The bacterial community diversities were analyzed by PCR-DGGE of the bacterial 16S rRNA gene. Results show approximately 60% BOD, 41% COD, 46% nitrate, 22% total nitrogen, and 97% coliforms were removed by this wetland system. DGGE profiles revealed the bacterial community diversities shifted progressively from the entry to the exit of both A and B systems in this wetland. The microbial populations in water, sediment, biofilms on plants, and soil were quite different from each others. The fecal indicator Escherichia coli was used as a marker to monitor the fecal contamination in all samples. From PCR-DGGE profiles, E. coli could be successfully removed by this wetland system. In conclusion, this constructed wetland is a very successful system for wastewater treatment and is able to remove most of the pollutants before they are discharged into KaoPing River. The results of this study provided useful suggestions for the government to assess the bacterial diversities and the efficiency of this wetland system, to protect people from hazardous risks, and to manage a constructed wetland in the future.
    Advisory Committee
  • Chih-Ming Kao - chair
  • Chan-Shing Lin - co-chair
  • Ssu-Ching Chen - co-chair
  • Jong-Kang Liu - advisor
  • Files
  • etd-0714108-114551.pdf
  • indicate in-campus access in a year and off_campus not accessible
    Date of Submission 2008-07-14

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