Title page for etd-0711117-105733


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URN etd-0711117-105733
Author Cheng-che Lu
Author's Email Address No Public.
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Department Biological Sciences
Year 2016
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title A Study of Cell-penetrating Peptides as a Delivery Vehicle in Cells of a Model Cnidarian
Date of Defense 2017-06-26
Page Count 38
Keyword
  • EGFP-ApHistone
  • cnidarian transfection
  • ApHistone
  • cell-penetrating peptides
  • COS7
  • Abstract Intracellular symbiosis between Symbiodinium dinoflagellates and corals forms coral reefs providing diverse and essential habitats for the existence of a large variety of marine organisms possible in the nutrient-poor tropical seas. However, our knowledge on the cellular and molecular mechanisms of this important ecological interaction is poor, partly due to a lack of a genetically manipulatable model of cnidarian. The aim of the present study is to establish a transfection system in a cnidarian model, Aiptasia pulchella, with cell-penetrating peptides (CPPs) as the delivery vehicle. I have successfully established a CPPs-based protocol to deliver pEGFP-ApHistone reporter plasmid into COS7 cells and expressed EGFP-ApHistone fusion protein in the nucleus. The results showed that the CPPs were able to penetrate A. pulchella cells and replaced the binding between DNA and Hoechst33258, as CPPs were not only penetrated into cells but also diffused into nucleus in the marine cnidarian cell model. Moreover, the results strongly suggests that CPPs carried the Cy3-conjugated DNA primers into the nucleus of A. pulcehlla endodermal cells, revealing its potentials for cell transfection reagents. Overall, all results provided evidence that the CPPs are one of transfection candidates for marine cnidarian even the efficiency was low under current protocol. Optimization of proceduces is underway to improve the delivery efficiency.
    Advisory Committee
  • Lee, Che-Hsin - chair
  • KH Chiu - co-chair
  • Chang, Hsueh-Wen - advisor
  • Files
  • etd-0711117-105733.pdf
  • Indicate in-campus at 5 year and off-campus access at 5 year.
    Date of Submission 2017-09-04

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