| URN |
etd-0711114-183817 |
|---|
| Author |
Siou-An Jhou |
|---|
| Author's Email Address |
No Public. |
|---|
| Statistics |
This thesis had been viewed 5570 times. Download 0 times. |
|---|
| Department |
Institute of Biomedical Sciences |
|---|
| Year |
2013 |
|---|
| Semester |
2 |
|---|
| Degree |
Master |
|---|
| Type of Document |
|
|---|
| Language |
English |
|---|
| Title |
A regularly spaced and self-revealing protein ladder for Western blot analysis |
|---|
| Date of Defense |
2014-07-24 |
|---|
| Page Count |
54 |
|---|
| Keyword |
protein marker
western blot
|
|---|
| Abstract |
Accurate determination of protein molecular weights is important for Western blot analysis(Towbin et al., 1979). Protein marker plays a very important role in the Western blot, it is composed of different molecular weight proteins to present bands on SDS PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) and western blot. According to different molecule weight band of the protein marker, investigators can estimate the molecule weight of protein sample and figure out if the sample is correct or not. In these experiments, investigators usually rely on pre-stained protein marker to analyze the protein sample(Parkinson and Redshaw, 1984) (Tsang et al., 1984). The advantages of the pre-stained protein marker is the band is stained with different color dye which can be clearly observed when we run the SDS PAGE gel. |
|---|
| Advisory Committee |
Bing-hong Chen - chair
Ming-Hong Tai - co-chair
Tian-Lu Cheng - advisor
|
|---|
| Files |
Indicate in-campus at 99 year and off-campus access at 99 year. |
|---|
| Date of Submission |
2014-08-12 |
|---|
