Title page for etd-0711114-183817


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URN etd-0711114-183817
Author Siou-An Jhou
Author's Email Address No Public.
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Department Institute of Biomedical Sciences
Year 2013
Semester 2
Degree Master
Type of Document
Language English
Title A regularly spaced and self-revealing protein ladder for Western blot analysis
Date of Defense 2014-07-24
Page Count 54
Keyword
  • protein marker
  • western blot
  • Abstract Accurate determination of protein molecular weights is important for Western blot analysis(Towbin et al., 1979). Protein marker plays a very important role in the Western blot, it is composed of different molecular weight proteins to present bands on SDS PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) and western blot. According to different molecule weight band of the protein marker, investigators can estimate the molecule weight of protein sample and figure out if the sample is correct or not. In these experiments, investigators usually rely on pre-stained protein marker to analyze the protein sample(Parkinson and Redshaw, 1984) (Tsang et al., 1984). The advantages of the pre-stained protein marker is the band is stained with different color dye which can be clearly observed when we run the SDS PAGE gel.
    Advisory Committee
  • Bing-hong Chen - chair
  • Ming-Hong Tai - co-chair
  • Tian-Lu Cheng - advisor
  • Files
  • etd-0711114-183817.pdf
  • Indicate in-campus at 99 year and off-campus access at 99 year.
    Date of Submission 2014-08-12

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