Title page for etd-0710103-100048


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URN etd-0710103-100048
Author Hung-Yi Chen
Author's Email Address m9021626@student.nsysu.edu.tw
Statistics This thesis had been viewed 5561 times. Download 8604 times.
Department Biological Sciences
Year 2002
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Analyzing the 16S rDNA to Monitor the Dynamic Microbial Communities in Petroleum Polluted Soil
Date of Defense 2003-07-04
Page Count 63
Keyword
  • 16S rDNA-DGGE
  • Abstract In this study, we had established a 16S rDNA-DGGE analys is system to detect the microbial community in petroleum polluted soil and assess the feasibility of using this system to monitor the bioremediation process. Three genomic DNA extracted methods, the KIT, the Bead-beating system, and the Freeze-thaw method were used
    to evaluate the DNA extraction efficiency and purity. These DNA samples were further tested by DGGE to analysis the microbial community in soil samples. The results showed that KIT method performed advantageous not only in the DNA extraction efficiency and purity, but also expressed the richest bacterial community in its
    PCR products. From the DGGE analysis, our data indicated that composition of bacterial community were different in the soil samples
    that were taken from the same site but at different time. This indicated that the species and number of microorganisms in a polluted soil were under a dynamic transition. The combination of DGGE and 16S rDNA gene sequence analysis system were also proven useful in identifying the predominant microbes in a soil sample and monitoring its bacterial community.
    Advisory Committee
  • none - chair
  • none - co-chair
  • JKL - advisor
  • Files
  • etd-0710103-100048.pdf
  • indicate in-campus access immediately and off_campus access in a year
    Date of Submission 2003-07-10

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