Title page for etd-0708113-160153


[Back to Results | New Search]

URN etd-0708113-160153
Author Chih-Hung Chou
Author's Email Address No Public.
Statistics This thesis had been viewed 5350 times. Download 67 times.
Department Chemistry
Year 2012
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Affinity directed preparation of protein macroinitiator and its application in the study of protein-polymer conjugates
Date of Defense 2013-07-22
Page Count 86
Keyword
  • PNIPAAm
  • N-isopropylacrylamide
  • Protein-polymer conjugates
  • ATRP
  • Tosyl
  • Abstract Protein-polymer conjugates are widely used in medicine, biotechnology, and methods to prepare the bioconjugates. In this research, a chemical method by using synthetic ligand-tosyl-initiator probes is proposed to prepare the protein macroinitiator and then used in the synthesis of protein-polymer conjugates. The ligand- tosyl chemical probes are composed of boronic acid and biotin as affinity ligands, a benzenesulfonyl group as a reactive group and an alkyl halide as the initiator in atom transfer radical polymerization (ATRP). Two proteins of interest, streptavidin and human IgG, were labeled with initiators via proximity effect; subsequently, the alkyl halide group can covalently transfer to the protein surface to obtain the stable macroinitiator. The obtained protein macroinitiator was then treated with N-isopropyl acrylamide in the presence of Cu (I) to furnish the desired protein-PNIPAAm conjugates. The resultant protein-polymer conjugates have been characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), dynamic light scattering (DLS), and reverse phase high-performance liquid chromatography (RP-HPLC).
    Advisory Committee
  • Chuan-Fa Chang - chair
  • Ming-Jung Wu - co-chair
  • Po-Shen Pan - co-chair
  • Po-Chiao Lin - advisor
  • Files
  • etd-0708113-160153.pdf
  • Indicate in-campus at 5 year and off-campus access at 5 year.
    Date of Submission 2013-08-22

    [Back to Results | New Search]


    Browse | Search All Available ETDs

    If you have more questions or technical problems, please contact eThesys