Title page for etd-0708108-123103


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URN etd-0708108-123103
Author Wan-yu Yang
Author's Email Address No Public.
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Department Biological Sciences
Year 2007
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Isolation and identification of fuel-oil-degrading bacteria
Date of Defense 2008-06-13
Page Count 121
Keyword
  • Biodegradation
  • Acinetobacter
  • Pseudomonas
  • DGGE
  • Crude oil
  • Abstract The purpose of this study is to isolate and identify the crude oil-degrading bacteria from oil polluted soil. Their physiological characteristics and oil-degrading capability were also studied. Eight polluted soil samples were taken from the Kaohsiung Refingery Factory of the Chinese Petroleum Corporation (CPC). The microbiota of the Kaohsiung refinery soil sample P37-2 (#6) could degrade crude oil from 2000 ppm to 572 ppm in 10 days. Bacteria in polluted soil samples were selected and isolated by minimal medium with 2000 ppm crude oil as the sole carbon source. Biochemical test, PCR-DGGE, and 16S DNA sequencing were used to identify and characterize the bacteria isolates. Three strains were identified as Pseudomonas aeruginosa (NSYSU-1-1), Acinetobacter sp. (NSYSU-4-1), and Pseudomonas sp. (NSYSU-7-1). These three strains and microbiota #6 were tested for their capability of degrading the total petroleum hydrocarbons (TPH). We found that microbiota #6 performed better than the other three bacterial strains in degrading the crude oil. In this study, we also found temperature was not the major factor of influcing the biodegradation; however, high oxygen concentration and providing nitrogen soure couled improve the biodegradation rate. Although both NSYSU-1-1 and NSYSU-7-1 are Pseudomonas strains, they performed different on degrading the oil. All strains tested could degrade the crude oil to a concentration below 1000 ppm to meet the government emission standard. The bacterial strains and techniques developed in this study provide a choice for future bioremediation of crude oil pollution.
    Advisory Committee
  • Jimmy C. M. Kao - chair
  • Chan-Shing Lin - co-chair
  • Ssu-Ching Chen - co-chair
  • Jong-Kang Liu - advisor
  • Files
  • etd-0708108-123103.pdf
  • indicate access worldwide
    Date of Submission 2008-07-08

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