Title page for etd-0704102-153004


[Back to Results | New Search]

URN etd-0704102-153004
Author Pei-Hsuan Lin
Author's Email Address oad@mail2000.com.tw
Statistics This thesis had been viewed 5350 times. Download 4219 times.
Department Institute of Biomedical Sciences
Year 2001
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Patch-clamp electrical recordings of rutaecarpine-induced block of delayed rectifier K current in NG-108-15 neuronal cells
Date of Defense 2002-06-24
Page Count 33
Keyword
  • rutaecarpine
  • patch-clamp
  • Abstract Abstract
    In the present study, the effects of rutaecarpine on ionic currents of NG108-15 neuronal cells were studied. Rutaecarpine (2-100 μM) suppressed the amplitude of voltage-dependent K+ outward current (IK(DR)) in a concentration-dependent manner. The IC50 value for rutaecarpine-induced inhibition of IK was 11μM. However, rutaecarpine (20 μM) had little effect on L-type Ca2+ current. IK(DR) present in these cells is sensitive to the inhibition by quinidine and dendrotoxin, yet not by E-4031. Rutaecarpine enhanced the rate and extent of IK(DR) inactivation, although it had no effect on the initial activation phase of IK(DR). Recovery from block by rutaecarpine (5 μM) was fitted by a single exponential with a value of 2.87 s. Cell-attached single-channel recordings revealed that rutaecarpine decreased channel activity over the length of the test potential without altering single-channel amplitude. With the aid of binding scheme, a quantitative description of the actions of rutaecarpine on IK(DR) was provided. Under current-clamp configuration, rutaecarpine also prolonged action potential duration in NG108-15 cells without altering other variables of the action potential. The results clearly show that rutaecarpine is a blocker of the KDR channel. The increase in action potential duration induced by rutaecarpine can be
    explained mainly by its blocking effects on IK(DR).
    Advisory Committee
  • Hung-Tu Huang - chair
  • Chung-Ren Jan - co-chair
  • Sheng-Nan Wu - advisor
  • Files
  • etd-0704102-153004.pdf
  • indicate in-campus access immediately and off_campus access in a year
    Date of Submission 2002-07-04

    [Back to Results | New Search]


    Browse | Search All Available ETDs

    If you have more questions or technical problems, please contact eThesys