Title page for etd-0701117-094143


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URN etd-0701117-094143
Author Meng-chia Liu
Author's Email Address No Public.
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Department Institute of Medical Science and Technology
Year 2016
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Light-triggered methylcellulose-gold nanoparticles hydrogel for leptin release to inhibit fat stores in adipocytes
Date of Defense 2017-07-20
Page Count 78
Keyword
  • gold nanoparticle
  • photothermal therapy
  • leptin
  • drug delivery
  • Methylcellulose
  • Abstract Leptin released in response to increased trigyceride storage in adipocytes, leptin appears to significantly affect body weight but suffer from poor therapeutic effect and side effects after systemic administration. Leptin modifies adipocytes sensitivity to insulin to inhibit lipid accumulation. Additionally, glucose uptake by adipose tissue found no response to leptin when leptin was administered intravenously or directly to adipocytes. This prolonged exposure to leptin did, however, cause a substantial dose dependent inhibition of insulin-stimulated glucose uptake by the adipocytes. Loading in light-triggered degradable hydrogels can offer improvements in the accuracy and efficacy for sustained delivery at specific sites. In our approach, leptin were entrapped with methylcellulose (MC)-based hydrogels, with incorporation of gold nanoparticles (NPs). The incorporation of gold NPs into the MC hydrogels led to a tunable light-irradiation response that dictated hydrogel release rate of leptin. This manuscript demonstrates feasibility in designing tunable-thermo-sensitive hydrogels for loading multimodality therapeutic agents to enhance the bioactivity of leptin for obesity therapy. In comparison with the experiment controls with doxorubicin only, the MC-gold NPs hydrogels significantly enhanced the rate of cell death via apoptosis at the additional 72 hour when illuminated at 980 nm for 10 min. The aim of this study is to develop a light-sensitive MC-H incorporated with gold nanoparticles (MC-gold NPs hydrogels) for localized leptin delivery.
    Advisory Committee
  • S-Ja Tseng - chair
  • Hsin-Jung Li - co-chair
  • Huang-Kuo Yen - co-chair
  • Zi-Xian Liao - advisor
  • Files
  • etd-0701117-094143.pdf
  • Indicate in-campus at 5 year and off-campus access at 5 year.
    Date of Submission 2017-08-01

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