Title page for etd-0701113-153125


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URN etd-0701113-153125
Author Jia-Yuan Li
Author's Email Address No Public.
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Department Biological Sciences
Year 2012
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Isolation and Characteristic Analysis of Mercury Resistant Bacteria
Date of Defense 2013-07-16
Page Count 121
Keyword
  • merA gene
  • real time PCR
  • mercuric reductase
  • DGGE
  • mercury
  • Abstract A combination of PCP, mercury and dioxin polluted site in Southern Taiwan causes great damage to the natural environment and endangers the health of nearby residents. The goal of this study is to isolate mercury resistant microorganisms from the polluted site and develop an in situ bioremediation technique. Because the concentration of mercury pollution in the polluted site is about 50 ppm, the medium used to isolate bacteria was designed to contain 60 ppm mercury ion. After mercury-resistant bacteria were isolated, PCR specific primers were applied to detect whether the bacteria contain merA gene or not. Identification of bacteria were based on their 16S rDNA sequences. In this study, three bacteria were isolated, designated as B37, A45 and A46. Species identification showed that B37 was closely related to Enterobacter cloacae while A45 and A46 were associated with Pseudomonas sp.. All three bacterial strains were also confirmed to grow well in 100 ppm Hg2+ media as well as under seawater salinity (3.5%). RT-PCR and real time PCR analysis revealed that the expression of merA gene could be induced by 10 ppm mercury ion in all 3 bacterial strains. Mercury ion removal batch studies exhibited that A45 strain in NB medium, combination of 3 strains in PMM medium, and B37 strain in LB mediums possessed the best treatment effect. After 12 days of incubation, 54.4%, 89.0% and 88.6% of mercury ions could be removed respectively. This study shows that these three bacterial strains are useful candidates in future bioremediation.
    Advisory Committee
  • Jimmy C. M. Kao - chair
  • Lei Yang - co-chair
  • Jong-Kang Liu - advisor
  • Files
  • etd-0701113-153125.pdf
  • Indicate in-campus at 99 year and off-campus access at 99 year.
    Date of Submission 2013-08-01

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