Title page for etd-0701113-151214


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URN etd-0701113-151214
Author Chao-chi Huang
Author's Email Address No Public.
Statistics This thesis had been viewed 5356 times. Download 823 times.
Department Mechanical and Electro-Mechanical Engineering
Year 2012
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title λDNA Hydrodynamics Stretching in Microchannels with Different Micropillar Arrays
Date of Defense 2013-07-24
Page Count 128
Keyword
  • DNA molecules
  • μLIF
  • electroosmotic flow
  • fluorescence microscope
  • μPIV
  • hydrodynamic stretching
  • Abstract In this study, electrokinetic-driven flow in microchannels with different types and sizes of micropillars was observed. There were parallel and staggered micropillars in types and 20 μm, 30 μm and 40 μm in diametral sizes. At last stretched λDNA was also observed. We utilized micrometer resolution particle image velocimetry (μPIV) to observe and calculate the field of velocity. And the field of temperature caused joule heating effect was detected by laser induced fluorescence (μLIF). Used fluorescence microscope (FM) recorded the image of DNA molecules. In the experiment, microchannels were produced by photo lithogrophy process which composed of two slices of PDMS after UV/Ozone. And different viscosities and intensity of electric field in 1 x TBE buffer solution were performed to gain corrected velocity, flow field, stretched DNA in microchannels with micropillars and so on.
    Advisory Committee
  • Ching-Jeng Ho - chair
  • Chin-Chia Su - co-chair
  • Chao-Kuang Chen - co-chair
  • Shou-Shing Hsieh - advisor
  • Files
  • etd-0701113-151214.pdf
  • indicate access worldwide
    Date of Submission 2013-08-01

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